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Research ArticleCELLULAR AND MOLECULAR

Green Tea Polyphenols Induce Differentiation and Proliferation in Epidermal Keratinocytes

Stephen Hsu, Wendy B. Bollag, Jill Lewis, Qin Huang, Baldev Singh, Mohamed Sharawy, Tetsuya Yamamoto and George Schuster
Journal of Pharmacology and Experimental Therapeutics July 2003, 306 (1) 29-34; DOI: https://doi.org/10.1124/jpet.103.049734
Stephen Hsu
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Wendy B. Bollag
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Jill Lewis
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Qin Huang
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Baldev Singh
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Mohamed Sharawy
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Tetsuya Yamamoto
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George Schuster
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Abstract

The most abundant green tea polyphenol, epigallocatechin-3-gallate (EGCG), was found to induce differential effects between tumor cells and normal cells. Nevertheless, how normal epithelial cells respond to the polyphenol at concentrations for which tumor cells undergo apoptosis is undefined. The current study tested exponentially growing and aged primary human epidermal keratinocytes in response to EGCG or a mixture of the four major green tea polyphenols. EGCG elicited cell differentiation with associated induction of p57/KIP2 within 24 h in growing keratinocytes, measured by the expression of keratin 1, filaggrin, and transglutaminase activity. Aged keratinocytes, which exhibited low basal cellular activities after culturing in growth medium for up to 25 days, renewed DNA synthesis and activated succinate dehydrogenase up to 37-fold upon exposure to either EGCG or the polyphenols. These results suggest that tea polyphenols may be used for treatment of wounds or certain skin conditions characterized by altered cellular activities or metabolism.

Footnotes

  • This study was supported by a grant from the Dental Research Foundation and funding through the Department of Oral Biology and Maxillofacial Pathology (School of Dentistry, Medical College of Georgia) (to S.H.).

  • DOI: 10.1124/jpet.103.049734.

  • ABBREVIATIONS: GTPP, green tea polyphenol; EGCG, epigallocatechin-3-gallate; KGM-2, keratinocyte growth medium-2; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; SDH, succinate dehydrogenase; BrdU, bromodeoxyuridine.

    • Received January 29, 2003.
    • Accepted March 25, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 306 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 306, Issue 1
1 Jul 2003
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Research ArticleCELLULAR AND MOLECULAR

Green Tea Polyphenols Induce Differentiation and Proliferation in Epidermal Keratinocytes

Stephen Hsu, Wendy B. Bollag, Jill Lewis, Qin Huang, Baldev Singh, Mohamed Sharawy, Tetsuya Yamamoto and George Schuster
Journal of Pharmacology and Experimental Therapeutics July 1, 2003, 306 (1) 29-34; DOI: https://doi.org/10.1124/jpet.103.049734

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Research ArticleCELLULAR AND MOLECULAR

Green Tea Polyphenols Induce Differentiation and Proliferation in Epidermal Keratinocytes

Stephen Hsu, Wendy B. Bollag, Jill Lewis, Qin Huang, Baldev Singh, Mohamed Sharawy, Tetsuya Yamamoto and George Schuster
Journal of Pharmacology and Experimental Therapeutics July 1, 2003, 306 (1) 29-34; DOI: https://doi.org/10.1124/jpet.103.049734
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