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Research ArticleCARDIOVASCULAR

Modification of Noradrenaline Release in Pithed Spontaneously Hypertensive Rats by I1-Binding Sites in Addition to α2-Adrenoceptors

Walter Raasch, Britta Jungbluth, Ulrich Schäfer, Walter Häuser and Peter Dominiak
Journal of Pharmacology and Experimental Therapeutics March 2003, 304 (3) 1063-1071; DOI: https://doi.org/10.1124/jpet.102.044966
Walter Raasch
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Britta Jungbluth
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Ulrich Schäfer
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Walter Häuser
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Peter Dominiak
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Abstract

It is known that moxonidine acts as an agonist at presynaptic α2-adrenoceptors of the postganglionic sympathetic nerve terminals and leads to a reduction in noradrenaline release. In addition, it is conceivable that I1-binding sites located in other regions of the pre- and postganglionic sympathetic neurons are involved in this effect. Our aim was to investigate whether and to what extent activation of the I1-binding sites contributes to the moxonidine-induced inhibition of noradrenaline release. Noradrenaline release was induced in pithed spontaneously hypertensive rats (pretreated with phenoxybenzamine/desipramine at 10/0.5 mg/kg) by stimulation of sympathetic overflow from the spinal cord. Noradrenaline overflow was reduced using moxonidine (0.18, 0.6, and 1.8 mg/kg) by 39.4, 70.4, or 78.7%, respectively, even when all α1-/α2-adrenoceptors were blocked effectively by phenoxybenzamine. In contrast, the I1-antagonist efaroxan (0.1, 1, and 3 mg/kg) increased noradrenaline overflow from 453 (control) to 1710, 1999, or 2754 pg/ml, suggesting an autoreceptor-like function of I1-binding sites. In consequence, moxonidine (0.18, 0.6, and 1.8 mg/kg) reduced the increase in noradrenaline overflow in efaroxan-treated animals (1 mg/kg) by 22.7, 41.7, and 50.5%, respectively. Agmatine (6 and 60 mg/kg), an endogenous agonist at I1-binding sites, reduced noradrenaline overflow (−36 or 53%), even under α2-adrenoceptor blockade. When 2-endo-amino-3-exo-isopropylbicyclo[2.2.1]heptane (AGN192403) (10 mg/kg) was injected, a selective blocker of I1-binding sites, noradrenaline overflow was not influenced by agmatine. It is concluded that moxonidine reduces noradrenaline overflow by acting at I1-binding sites in addition to its agonistic property at α2-adrenoceptors. The exact location of the I1-binding sites on the pre- or postsynaptic sympathetic neurons is unknown, but the location in the pre- or postsynaptic membrane of the sympathetic ganglion is the most plausible explanation.

Footnotes

  • DOI: 10.1124/jpet.102.044966

  • Abbreviations:
    SHR
    spontaneously hypertensive rats
    KO
    knockout
    PE
    polyethylene
    nAch
    nicotinic acetylcholine
    • Received October 1, 2002.
    • Accepted November 22, 2002.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 304 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 304, Issue 3
1 Mar 2003
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Research ArticleCARDIOVASCULAR

Modification of Noradrenaline Release in Pithed Spontaneously Hypertensive Rats by I1-Binding Sites in Addition to α2-Adrenoceptors

Walter Raasch, Britta Jungbluth, Ulrich Schäfer, Walter Häuser and Peter Dominiak
Journal of Pharmacology and Experimental Therapeutics March 1, 2003, 304 (3) 1063-1071; DOI: https://doi.org/10.1124/jpet.102.044966

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Research ArticleCARDIOVASCULAR

Modification of Noradrenaline Release in Pithed Spontaneously Hypertensive Rats by I1-Binding Sites in Addition to α2-Adrenoceptors

Walter Raasch, Britta Jungbluth, Ulrich Schäfer, Walter Häuser and Peter Dominiak
Journal of Pharmacology and Experimental Therapeutics March 1, 2003, 304 (3) 1063-1071; DOI: https://doi.org/10.1124/jpet.102.044966
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