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Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Effect of β3-Adrenoceptor Agonist in Lower Esophageal Sphincter Smooth Muscle: In Vitro Studies

D. N. K. Sarma, Kuldip Banwait, Ashim Basak, Anthony J. DiMarino and Satish Rattan
Journal of Pharmacology and Experimental Therapeutics January 2003, 304 (1) 48-55; DOI: https://doi.org/10.1124/jpet.102.040501
D. N. K. Sarma
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Kuldip Banwait
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Ashim Basak
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Anthony J. DiMarino
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Satish Rattan
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Abstract

We investigated the effects of (R,R)-5-[2-[2–3-chlorophenyl)-2-hydroxyethyl] - amino]propyl] - 1,3 - benzodioxole - 2 , 2 - dicarboxylate (CL 316243) (a typical β3-agonist) on the spontaneously tonic smooth muscle of the lower esophageal sphincter (LES). Studies were carried out in smooth muscle strips and smooth muscle cells (SMCs) of opossum LES. Isometric tension was recorded in the basal state and after CL 316243, and before and after β3-antagonist (S)-N-[4-[2-[[3-[-(acetamidomethyl)phenoxy]-2-hydroxypropyl]amino]ethyl]phenyl]benzenesulfonamide (L 748337) and nonselective antagonist propranolol. In some experiments, the effects of nonadrenergic noncholinergic (NANC) nerve activation by electrical field stimulation (EFS) were also examined. The effects of CL 316243 were compared with those of nonselective β-agonist isoproterenol. CL 316243 caused a concentration-dependent relaxation of the LES smooth muscle. The relaxant action of CL 316243 was determined to be directly at the smooth muscle because it remained unmodified by the neurotoxin tetrodotoxin and other neurohumoral antagonists, and also was observed in the SMCs. L 748337 selectively antagonized the relaxant effect of CL 316243 and, conversely, had no significant effect on the inhibitory actions of isoproterenol. CL 316243 (1 × 10−8 M) caused an augmentation of NANC relaxation in the LES. Another β3-agonist, (S)-4-[hydroxy-3-phenoxy-propylamino-ethoxy]-N-(2-methoxyethyl)-phenoxyacetamide (ZD 7114), also caused concentration-dependent full relaxation of the LES that was selectively antagonized by β3-anatagonist 3-(2-ethylphenoxy)-1-[(1S)1,2,3,4-tetrahydronaphth-1-ylaminol]-(2S)-2-propanol oxalate (SR 59230A). These studies defined the effects of characteristic inhibitory β3-adrenoceptors in the spontaneously tonic LES smooth muscle and suggested a potential therapeutic role in the esophageal motility disorders characterized by hypertensive LES.

Footnotes

  • This study was supported by National Institutes of Diabetes and Digestive and Kidney Diseases Grant DK-35385 and an institutional grant from Thomas Jefferson University, Philadelphia, PA.

  • DOI: 10.1124/jpet.102.040501

  • Abbreviations:
    LES
    lower esophageal sphincter
    SMS
    smooth muscle cell
    EFS
    electrical field stimulation
    NANC
    nonadrenergic noncholinergic
    CRC
    concentration-response curve
    CL 316243
    disodium (R,R)-5-[2-[2–3-chlorophenyl)-2-hydroxyethyl]-amino]propyl]-1,3-benzodioxole-2,2-dicarboxylate
    SR 59230A
    3-(2-ethylphenoxy)-1-[(1S)1,2,3,4-tetrahydronaphth-1-ylaminol]-(2S)-2-propanol oxalate
    L 748337
    (S)-N-[4-[2-[[3-[-(acetamidomethyl)phenoxy]-2-hydroxypropyl]amino]ethyl]phenyl]benzenesulfonamide
    TTX
    tetrodotoxin
    ZD 7114
    (S)-4-[hydroxy-3-phenoxy-propylamino-ethoxy]-N-(2-methoxyethyl)-phenoxyacetamide
    • Received June 18, 2002.
    • Accepted July 22, 2002.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 304 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 304, Issue 1
1 Jan 2003
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Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Effect of β3-Adrenoceptor Agonist in Lower Esophageal Sphincter Smooth Muscle: In Vitro Studies

D. N. K. Sarma, Kuldip Banwait, Ashim Basak, Anthony J. DiMarino and Satish Rattan
Journal of Pharmacology and Experimental Therapeutics January 1, 2003, 304 (1) 48-55; DOI: https://doi.org/10.1124/jpet.102.040501

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Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Effect of β3-Adrenoceptor Agonist in Lower Esophageal Sphincter Smooth Muscle: In Vitro Studies

D. N. K. Sarma, Kuldip Banwait, Ashim Basak, Anthony J. DiMarino and Satish Rattan
Journal of Pharmacology and Experimental Therapeutics January 1, 2003, 304 (1) 48-55; DOI: https://doi.org/10.1124/jpet.102.040501
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