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Research ArticleCELLULAR AND MOLECULAR

Direct Examination of Local Regulation of Membrane Activity in Striatal and Prefrontal Cortical Neurons in Vivo Using Simultaneous Intracellular Recording and Microdialysis

A. R. West, H. Moore and A. A. Grace
Journal of Pharmacology and Experimental Therapeutics June 2002, 301 (3) 867-877; DOI: https://doi.org/10.1124/jpet.301.3.867
A. R. West
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H. Moore
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A. A. Grace
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Abstract

Slice preparations are typically used to study the effects of pharmacological manipulations on the electrophysiological activity of mature neurons. However, the severing of afferent inputs is known to significantly change the natural membrane activity of the neuron. To study the effects of local pharmacological manipulations on neurons in the intact brain, we combined the methods of microdialysis and intracellular recording in vivo. After implantation of a microdialysis probe into the prefrontal cortex (PFC) or striatum, intracellular recordings were conducted within ∼500 μm of the active surface of the probe. The spontaneous membrane activity, passive membrane properties, and intracellularly and synaptically evoked responses of striatal and cortical neurons recorded during perfusion of artificial cerebral spinal fluid were not different from that of neurons recorded in intact animals. Moreover, in the PFC, local perfusion with glutamate orN-methyl-d-aspartate depolarized neurons and increased spike activity. Conversely, local perfusion of tetrodotoxin hyperpolarized neurons while markedly reducing spontaneous membrane depolarizations and eliminating spike activity. In the striatum, local perfusion of the γ-aminobutyric acidA receptor antagonist bicuculline rapidly depolarized neurons and increased spontaneous spike activity. Given that striatal and PFC neurons recorded in animals undergoing microdialysis in the current study exhibited electrophysiological properties similar to those recorded in intact controls, it is likely that the effects of local microdialysis on ongoing synaptic activity, neuronal excitability, and endogenous neurotransmitter levels are minimal. We conclude that the use of local microdialysis with intracellular recording is a powerful method for studying local receptor regulation of synaptic activity in vivo.

Footnotes

  • This study was supported by U.S. Public Health Service Grants MH-45156 and 57440 (to A.A.G.); National Research Service awards (to A.R.W. and H.M.); and grants from the Tourette Syndrome Association (to A.R.W.), the National Alliance for Research on Schizophrenia and Depression (to A.R.W.), and Stanley Foundation (to H.M. and A.A.G.).

  • Abbreviations:
    aCSF
    artifical cerebrospinal fluid
    GABA
    γ-aminobutyric acid
    TTX
    tetrodotoxin
    BIC
    bicuculline
    NMDA
    N-methyl-d-aspartate
    PFC
    prefrontal cortex
    PB
    phosphate buffer
    PBS
    phosphate-buffered saline
    TBS
    Tris-buffered saline
    EPSP
    excitatory postsynaptic potential
    IR
    input resistance
    • Received December 14, 2001.
    • Accepted February 25, 2002.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 301 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 301, Issue 3
1 Jun 2002
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Research ArticleCELLULAR AND MOLECULAR

Direct Examination of Local Regulation of Membrane Activity in Striatal and Prefrontal Cortical Neurons in Vivo Using Simultaneous Intracellular Recording and Microdialysis

A. R. West, H. Moore and A. A. Grace
Journal of Pharmacology and Experimental Therapeutics June 1, 2002, 301 (3) 867-877; DOI: https://doi.org/10.1124/jpet.301.3.867

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Research ArticleCELLULAR AND MOLECULAR

Direct Examination of Local Regulation of Membrane Activity in Striatal and Prefrontal Cortical Neurons in Vivo Using Simultaneous Intracellular Recording and Microdialysis

A. R. West, H. Moore and A. A. Grace
Journal of Pharmacology and Experimental Therapeutics June 1, 2002, 301 (3) 867-877; DOI: https://doi.org/10.1124/jpet.301.3.867
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