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Research ArticleENDOCRINE AND REPRODUCTIVE

Increase in Neurokinin B Expression and in Tachykinin NK3 Receptor-Mediated Response and Expression in the Rat Uterus with Age

Cristina G. Cintado, Francisco M. Pinto, Philippe Devillier, Angel Merida and M. Luz Candenas
Journal of Pharmacology and Experimental Therapeutics December 2001, 299 (3) 934-938;
Cristina G. Cintado
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Francisco M. Pinto
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Philippe Devillier
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Angel Merida
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M. Luz Candenas
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Abstract

We analyzed tachykinin NK3 receptor (NK3R) gene expression by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in uteri from young (3-month-old) and old (30-month-old) rats. In addition, we characterized the expression of the preprotachykinin-B (TAC-3) gene, which encodes neurokinin B (NKB), the preferred endogenous agonist of NK3R. Compared with young rats, NK3R messenger RNA (mRNA) levels were about 45-fold higher in uteri from old animals. TAC-3 mRNA was expressed in the rat uterus, and its levels were about 2.5-fold higher in old than in young rats. The contractile effect of the selective tachykinin NK3R agonist [MePhe7]-NKB in uteri from young and old animals was investigated by using conventional organ bath technique. A marked correlation was observed between the magnitude of the contraction elicited by [MePhe7]-NKB and the level of expression determined by RT-PCR for the NK3R. These observations are consistent with a role for the NKB/NK3R ligand-receptor pair in regulating uterine functions and support the existence of a link between estrogen and the NK3R/NKB activation pathway.

Footnotes

  • ↵1 Recipient of a fellowship from the Ministry of Science and Technology (Spain).

  • This work was supported by Grant PB 97-1123 from the Ministry of Science and Technology (Spain).

  • Abbreviations:
    SP
    substance P
    NKB
    neurokinin B
    NKA
    neurokinin A
    NK1R
    tachykinin NK1 receptor
    NK2R
    tachykinin NK2 receptor
    NK3R
    tachykinin NK3 receptor
    RT-PCR
    reverse transcription-polymerase chain reaction
    bp
    base pairs
    GAPDH
    glyceraldehyde-3-phosphate dehydrogenase
    PP1-δ
    protein phosphatase type 1 δ-isoform
    ACh
    acetylcholine
    • Received June 7, 2001.
    • Accepted August 20, 2001.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 299 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 299, Issue 3
1 Dec 2001
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Research ArticleENDOCRINE AND REPRODUCTIVE

Increase in Neurokinin B Expression and in Tachykinin NK3 Receptor-Mediated Response and Expression in the Rat Uterus with Age

Cristina G. Cintado, Francisco M. Pinto, Philippe Devillier, Angel Merida and M. Luz Candenas
Journal of Pharmacology and Experimental Therapeutics December 1, 2001, 299 (3) 934-938;

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Research ArticleENDOCRINE AND REPRODUCTIVE

Increase in Neurokinin B Expression and in Tachykinin NK3 Receptor-Mediated Response and Expression in the Rat Uterus with Age

Cristina G. Cintado, Francisco M. Pinto, Philippe Devillier, Angel Merida and M. Luz Candenas
Journal of Pharmacology and Experimental Therapeutics December 1, 2001, 299 (3) 934-938;
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