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Research ArticleCARDIOVASCULAR

Presynaptic α7-Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Nitric Oxidergic Neurogenic Vasodilation in Porcine Basilar Arteries

Min-Liang Si and Tony J. F. Lee
Journal of Pharmacology and Experimental Therapeutics July 2001, 298 (1) 122-128;
Min-Liang Si
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Tony J. F. Lee
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Abstract

We previously reported that nicotine-induced nitric oxide (NO)-mediated neurogenic vasodilation in the porcine basilar artery was dependent on intact sympathetic innervation. We further demonstrated in this artery that nicotine acted on nicotinic acetylcholine receptors (nAChRs) on presynaptic sympathetic nerve terminals to release norepinephrine (NE), which then acted on β2-adrenoceptors located on the neighboring NOergic nerve terminals to release NO, resulting in vasodilation. The nature of the nAChRs has not been determined. The nAChR subtype mediating nicotine-induced dilation in isolated porcine basilar arterial rings denuded of endothelium was therefore examined pharmacologically and immunohistochemically. Results from using an in vitro tissue bath technique indicated that relaxation induced by nicotine (100 μM) was blocked by preferential α7-nAChR antagonists (methyllycaconitine and α-bungarotoxin) and nonspecific nAChR antagonist (mecamylamine) in a concentration-dependent manner, but was not affected by dihydro-β-erythroidine (a preferential α4-nAChR antagonist). These nAChR antagonists did not affect relaxation elicited by transmural nerve stimulation (8 Hz) or that by sodium nitroprusside and NE. Results from double-labeling immunohistochemical studies in whole-mount porcine basilar and middle cerebral arteries and in cultured porcine superior cervical ganglia (SCG) indicated that α7-nAChR- and tyrosine hydroxylase immunoreactivities were colocalized in same nerve fibers. These results suggest the presence of functional α7-nAChRs on postganglionic sympathetic adrenergic nerve terminals of SCG origin, which mediate nicotine-induced neurogenic NOergic vasodilation. These findings are consistent with our hypothesis that nicotine acts on nAChRs on presynaptic sympathetic nerve terminals to release NE, which then acts on presynaptic β2-adrenoceptors located on the neighboring NOergic nerve terminals, resulting in release of NO and dilation of porcine basilar arteries.

Footnotes

  • This work was supported by National Institutes of Health HL 27763 and HL 47574, AHA/IHA (9807871), and SIU-CRC/EAM.

  • Abbreviations:
    nAChR
    nicotinic acetylcholine receptor
    NE
    norepinephrine
    NO
    nitric oxide
    TNS
    transmural nerve stimulation
    l-NNA
    N-nitro-l-arginine
    SCG
    superior cervical ganglion
    TTX
    tetrodotoxin
    PBS
    phosphate-buffered saline
    FITC
    fluorescein isothiocyanate
    TRITC
    tetramethylrhodamine isothiocyanate
    MLA
    methyllycaconitine
    α-BGTX
    α-bungarotoxin
    DHβE
    dihydro-β-erythroidine
    • Received January 16, 2001.
    • Accepted April 9, 2001.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 298 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 298, Issue 1
1 Jul 2001
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Research ArticleCARDIOVASCULAR

Presynaptic α7-Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Nitric Oxidergic Neurogenic Vasodilation in Porcine Basilar Arteries

Min-Liang Si and Tony J. F. Lee
Journal of Pharmacology and Experimental Therapeutics July 1, 2001, 298 (1) 122-128;

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Research ArticleCARDIOVASCULAR

Presynaptic α7-Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Nitric Oxidergic Neurogenic Vasodilation in Porcine Basilar Arteries

Min-Liang Si and Tony J. F. Lee
Journal of Pharmacology and Experimental Therapeutics July 1, 2001, 298 (1) 122-128;
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