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Research ArticleNEUROPHARMACOLOGY

Correlation between the Release of the Sympathetic Neurotransmitter ATP and Soluble Nucleotidases from the Guinea Pig Vas Deferens

Svetlana Mihaylova-Todorova, Latchezar D. Todorov and David P. Westfall
Journal of Pharmacology and Experimental Therapeutics January 2001, 296 (1) 64-70;
Svetlana Mihaylova-Todorova
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Latchezar D. Todorov
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David P. Westfall
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Abstract

Recently, we have shown that by releasing specific nucleotidases the sympathetic nerves of the guinea pig vas deferens may regulate the metabolism of extracellular adenine nucleotides and consequently, the inactivation of neurotransmitter ATP. Based on the evidence for tetrodotoxin sensitivity and calcium dependence of the nerve stimulation-evoked overflow of enzyme activity, we have suggested that soluble nucleotidases may be stored in synaptic vesicles within the sympathetic nerves and released upon arrival of nerve action potentials by a mechanism similar to that for release of neurotransmitters. To further test this hypothesis we studied the time course of nerve stimulation-evoked overflow of ATP, norepinephrine (NE), releasable ATPase (r-ATPase) activity, and releasable AMPase (r-AMPase) activity under control conditions and in the presence of drugs known to selectively modulate sympathetic neurotransmission. The results show that the time course of overflow of r-ATPase and r-AMPase activities resembles the transient pattern of overflow of ATP but not the tonic pattern of overflow of NE. Vasa deferentia dissected from animals treated with reserpine release ATP, r-ATPase, and r-AMPase, whereas the overflow of NE is completely abolished. Guanethidine, on the other hand, inhibits equally well the overflow of the two neurotransmitters and the releasable nucleotidase activities. Agonists of the α2-adrenergic receptors abolish the overflow of ATP, r- ATPase, and r-AMPase but not the overflow of NE. This evidence supports the idea that the sympathetic nerves of the guinea pig vas deferens store and release ATP together with specific nucleotidases responsible for the inactivation of this neurotransmitter.

Footnotes

  • Send reprint requests to: Svetlana Mihaylova-Todorova, M.D., Ph.D., Department of Pharmacology, University of Nevada School of Medicine, Howard Medical Sciences Bldg. Rm. 222, MS 318, Reno, NV 89557-0046. E-mail: mihay_s{at}med.unr.edu

  • This work was supported by Grant HL 38126 from the National Institutes of Health. A version of this work has appeared as a proceeding article to the Second International Workshop on Ecto-ATPases and Related Ectonucleotidases, Diepenbeek, Belgium, June 14–18, 1999 (see Mihaylova-Todorova et al., 2000a).

  • Abbreviations:
    E-NTPDase
    ectonucleoside triphosphate diphosphohydrolase
    ADO
    adenosine
    α,β-m ADP
    α,β-methylene 5′-adenosine diphosphate
    ATPDase
    adenosine tri-di-phosphatase, also apyraser-ATPase
    releasable ATPase
    r-ATPase
    NE
    norepinephrine
    r-AMPase
    releasable AMPase
    EFS
    electrical field stimulation
    eATP
    1N6-etheno-ATP
    eADP
    1N6-etheno-ADP
    eAMP
    1N6-etheno-AMP
    eADO
    1N6-etheno-ADO
    PUR
    sum total of ATP + ADP + AMP + ADO
    • Received May 10, 2000.
    • Accepted July 28, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 296 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 296, Issue 1
1 Jan 2001
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Research ArticleNEUROPHARMACOLOGY

Correlation between the Release of the Sympathetic Neurotransmitter ATP and Soluble Nucleotidases from the Guinea Pig Vas Deferens

Svetlana Mihaylova-Todorova, Latchezar D. Todorov and David P. Westfall
Journal of Pharmacology and Experimental Therapeutics January 1, 2001, 296 (1) 64-70;

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Research ArticleNEUROPHARMACOLOGY

Correlation between the Release of the Sympathetic Neurotransmitter ATP and Soluble Nucleotidases from the Guinea Pig Vas Deferens

Svetlana Mihaylova-Todorova, Latchezar D. Todorov and David P. Westfall
Journal of Pharmacology and Experimental Therapeutics January 1, 2001, 296 (1) 64-70;
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