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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Right Heart Failure Impairs Hepatic Elimination of p-Nitrophenol without Inducing Changes in Content or Latency of Hepatic UDP-Glucuronosyltransferases

Connie Y. Ng, Hany Ghabrial, Denis J. Morgan, Michael S. Ching, Richard A. Smallwood and Peter W. Angus
Journal of Pharmacology and Experimental Therapeutics November 2000, 295 (2) 830-835;
Connie Y. Ng
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Hany Ghabrial
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Denis J. Morgan
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Michael S. Ching
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Richard A. Smallwood
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Peter W. Angus
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Abstract

Congestive heart failure has been shown to affect oxidative drug metabolism, however, there has been little study of its effects on drug conjugation. Using the isolated perfused livers from rats with right ventricular failure (RVF) due to pulmonary artery constriction, we studied the effects of RVF on hepatic elimination ofp-nitrophenol (PNP) under controlled flow and oxygen delivery conditions. Hepatic clearance of the drug was found to be significantly impaired in RVF as compared with the sham group (0.80 ± 0.23 versus 1.28 ± 0.26 ml/min/g of liver). The impairment of PNP clearance in RVF occurred in parallel with significant reduction in metabolic formation clearance ofp-nitrophenyl-β-d-glucuronide; the major metabolite of PNP (0.51 ± 0.12 versus 1.03 ± 0.26 ml/min/g of liver). The intrinsic drug-glucuronidation capacity of livers was evaluated by measuring the microsomal content and activity of the UDP-glucuronosyltransferase(s) (UDP-GT) towardp-nitrophenol. There was no significant difference between sham and the RVF groups in either the content or the activity of the UDP-GT. The latency of the UDP-GT enzymes in microsomes was measured and was found to be similar between the two groups. The results of this study show that RVF impairs hepatic elimination of PNP and that this appears to be independent of changes in hepatic perfusion and oxygenation or alterations in hepatic content, activity, and latency of the UDP-GT.

Footnotes

  • Send reprint requests to: Peter W. Angus, University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Repatriation Campus, Heidelberg West, Victoria 3081, Australia. E-mail: pangus{at}patash.com.au

  • ↵1 This study was supported by the Australian National Health and Medical Research Council.

  • Abbreviations:
    RVF
    right ventricular failure
    IPRL
    isolated perfused rat liver
    PAC
    pulmonary artery constriction
    PLPC
    palmitoyl-lysophosphatidylcholine
    PNP
    p-nitrophenol
    PNPG
    p-nitrophenyl-β-d-glucuronide
    UDP-GA
    uridine diphosphoglucuronic acid
    UDP-GT
    uridine diphosphate-glucuronosyltransferase(s)
    • Received November 12, 1999.
    • Accepted July 20, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 295 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 295, Issue 2
1 Nov 2000
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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Right Heart Failure Impairs Hepatic Elimination of p-Nitrophenol without Inducing Changes in Content or Latency of Hepatic UDP-Glucuronosyltransferases

Connie Y. Ng, Hany Ghabrial, Denis J. Morgan, Michael S. Ching, Richard A. Smallwood and Peter W. Angus
Journal of Pharmacology and Experimental Therapeutics November 1, 2000, 295 (2) 830-835;

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Research ArticleABSORPTION, DISTRIBUTION, METABOLISM, AND EXCRETION

Right Heart Failure Impairs Hepatic Elimination of p-Nitrophenol without Inducing Changes in Content or Latency of Hepatic UDP-Glucuronosyltransferases

Connie Y. Ng, Hany Ghabrial, Denis J. Morgan, Michael S. Ching, Richard A. Smallwood and Peter W. Angus
Journal of Pharmacology and Experimental Therapeutics November 1, 2000, 295 (2) 830-835;
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