Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleCARDIOVASCULAR

Diadenosine Polyphosphates Cause Contraction and Relaxation in Isolated Rat Resistance Arteries

Martin Steinmetz, Eberhard Schlatter, Harry Alexander Jozef Struijker Boudier, Karl Heinz Rahn and Jozef Gabriel Rita De Mey
Journal of Pharmacology and Experimental Therapeutics September 2000, 294 (3) 1175-1181;
Martin Steinmetz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eberhard Schlatter
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Harry Alexander Jozef Struijker Boudier
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karl Heinz Rahn
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jozef Gabriel Rita De Mey
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The effects of diadenosine polyphosphates (APnA; n= 3–6) and adenine nucleotides on contractile reactivity of isolated rat mesenteric resistance arteries (MrA) and superior epigastric arteries (SEA), which display a dense and sparse autonomic innervation, respectively, were evaluated. All agonists examined, except adenosine and AMP, induced contractions. The rank order of potency was similar in both arteries: α,β-methylene ATP (α,β-meATP) > AP5A > AP6A > AP4A > ATP > ADP > AP3A. Contractions were stable during several minutes in SEA but highly transient in MrA. They were reduced after exposure to 10 μM α,β-meATP and by 10 μM of the P2X antagonist pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid. During phenylephrine (10 μM)-induced contractions, the agonists induced a further contraction in SEA. In MrA, however, further contraction was followed by marked relaxation. The rank order of relaxing potency was comparable to that of the contractile potency of agonists. Also, the relaxing effects of APnA were blunted by 10 μM pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid and after exposure to α,β-meATP. In vitro and in vivo sympathectomy with 6-hydroxydopamine and removal of the endothelium did not modify the effects of APnA in MrA. Thus, the contractile effects of APnA in resistance arteries 1) are due to a P2X purinoceptor-mediated stimulation of the smooth muscle; 2) depend on the length of the phosphate chain; and 3) are followed by endothelium-independent relaxing effects in MrA but not SEA, which may involve receptors that are similar to those mediating contraction. The regional heterogeneity of APnA effects cannot be attributed to a direct neurogenic influence.

Footnotes

  • Send reprint requests to: Dr. M. Steinmetz, Medizinische Poliklinik, Universität Münster, Albert-Schweitzer Strasse 33, 48129 Münster, Germany. E-mail:steinme{at}uni-muenster.de

  • ↵1 This study was supported by a grant of the Center for Interdisciplinary Clinical Research (IZKF, project A1) at the Medical Faculty of the University of Münster (BMBF 01 KS 9604/0) and the Cardiovascular Research Institute Maastrich at the University of Maastricht.

  • Abbreviations:
    APnA
    diadenosine polyphosphates
    MrA
    mesenteric resistance artery
    SEA
    superior epigastric artery
    6-OHDA
    6-hydroxydopamine
    α,β-me ATP
    α,β-methylene ATP
    PPADS
    pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid
    KRB
    Krebs-Ringer-bicarbonate solution
    • Received February 14, 2000.
    • Accepted May 4, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 294 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 294, Issue 3
1 Sep 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Diadenosine Polyphosphates Cause Contraction and Relaxation in Isolated Rat Resistance Arteries
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleCARDIOVASCULAR

Diadenosine Polyphosphates Cause Contraction and Relaxation in Isolated Rat Resistance Arteries

Martin Steinmetz, Eberhard Schlatter, Harry Alexander Jozef Struijker Boudier, Karl Heinz Rahn and Jozef Gabriel Rita De Mey
Journal of Pharmacology and Experimental Therapeutics September 1, 2000, 294 (3) 1175-1181;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleCARDIOVASCULAR

Diadenosine Polyphosphates Cause Contraction and Relaxation in Isolated Rat Resistance Arteries

Martin Steinmetz, Eberhard Schlatter, Harry Alexander Jozef Struijker Boudier, Karl Heinz Rahn and Jozef Gabriel Rita De Mey
Journal of Pharmacology and Experimental Therapeutics September 1, 2000, 294 (3) 1175-1181;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Optimized S-nitrosohemoglobin synthesis in red blood cells
  • High-Salt Diet Upregulates CaSR Expression and Signaling
  • L-Arginine improves post-infarction physical function
Show more Cardiovascular

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics