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Research ArticleNEUROPHARMACOLOGY

Evidence for Y1-Receptor-Mediated Facilitatory, Modulatory Cotransmission by NPY in the Rat Anococcygeus Muscle

Yolanda Hoyo, John C. McGrath and Elisabet Vila
Journal of Pharmacology and Experimental Therapeutics July 2000, 294 (1) 38-44;
Yolanda Hoyo
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John C. McGrath
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Elisabet Vila
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Abstract

The potential role of neuropeptide Y (NPY) as a neuromodulatory cotransmitter was investigated in rat anococcygeus muscle. The effects of NPY on contraction to norepinephrine or adrenergic nerve stimulation and on relaxation to nonadrenergic, noncholinergic nerve stimulation were analyzed. Norepinephrine-induced contraction was enhanced by NPY (0.1 μM). The Y1 receptor antagonist BIBP 3226 (1 μM) completely reversed this effect. NPY (0.01 or 0.1 μM) increased contractions induced by electrical field stimulation of sympathetic nerves. This increase was reduced by BIBP 3226 (1 μM), indicating Y1 receptor involvement. NPY (13–36) a Y2 receptor agonist, at 0.1 μM but not 0.01 μM, caused an increase of the nerve-induced contraction, which was reversed by BIBP 3226 (1 μM), indicating no Y2 receptor involvement. BIBP 3226 (1–1 μM) produced a concentration-dependent attenuation of nerve-mediated but not norepinephrine-mediated contraction. The reduction in nonadrenergic, noncholinergic nerve-induced relaxation to nerve stimulation by NPY (0.1 μM) was not affected by BIBP 3226 (1 μM). It is concluded that 1) exogenous NPY increases excitatory nerve-induced contraction mainly via a Y1 receptor-mediated effect on smooth muscle with a small non-Y1 receptor component due to blocking inhibitory nitrergic nerves and 2) endogenous NPY is a modulatory cotransmitter, which facilitates the primarily noradrenergic contractile responses to sympathetic nerve stimulation via smooth muscle Y1 receptors.

Footnotes

  • Send reprint requests to: Dr. Elisabet Vila, Departament de Farmacologia i Terapèutica, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain. E-mail:elisabet.vila{at}uab.es

  • ↵1 This work was supported by Dirección General de Investigación Cientifica y Técnica (PM95-0124), Acciones Integradas (HB1998-0146), and Grups de Qualitat de recerca (1999SGR-00119).

  • Abbreviations:
    NPY
    neuropeptide Y
    NANC
    nonadrenergic, noncholinergic
    TTX
    tetrodotoxin
    • Received October 27, 1999.
    • Accepted March 29, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 294 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 294, Issue 1
1 Jul 2000
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Research ArticleNEUROPHARMACOLOGY

Evidence for Y1-Receptor-Mediated Facilitatory, Modulatory Cotransmission by NPY in the Rat Anococcygeus Muscle

Yolanda Hoyo, John C. McGrath and Elisabet Vila
Journal of Pharmacology and Experimental Therapeutics July 1, 2000, 294 (1) 38-44;

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Research ArticleNEUROPHARMACOLOGY

Evidence for Y1-Receptor-Mediated Facilitatory, Modulatory Cotransmission by NPY in the Rat Anococcygeus Muscle

Yolanda Hoyo, John C. McGrath and Elisabet Vila
Journal of Pharmacology and Experimental Therapeutics July 1, 2000, 294 (1) 38-44;
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