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Research ArticleTOXICOLOGY

Effect of Oral and IntravenousS,N-Diacetylcysteine Monoethyl Ester on Circulating and Hepatic Sulfhydryls in the Rat

Ignazio Grattagliano, Gianluigi Vendemiale, Veronika B. Deiss, Edith Junker and Bernhard H. Lauterburg
Journal of Pharmacology and Experimental Therapeutics July 2000, 294 (1) 155-159;
Ignazio Grattagliano
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Gianluigi Vendemiale
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Veronika B. Deiss
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Edith Junker
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Bernhard H. Lauterburg
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Abstract

The role of GSH in the detoxification of reactive metabolites of oxygen and xenobiotics, in gene expression, and as a source of cysteine is well established. Because decreased circulating and intracellular concentrations of GSH might be of pathogenetic relevance in several clinical conditions, there is a growing interest in pharmacological interventions to correct a deranged sulfhydryl status. In this study, the disposition and the effect ofS,N-diacetylcysteine monoethyl ester (DACE) on sulfhydryls were investigated after i.v. and intraduodenal (i.d.) administrations to rats. DACE was rapidly hydrolyzed and deacetylated to N-acetylcysteine and cysteine in plasma. High concentrations of cysteine were attained in the circulation and in the liver after i.v. and i.d. administrations of 5 mmol/kg DACE, and physiological levels of GSH in the liver and in plasma increased by 30 and 300%, respectively, with i.v. and i.d. administrations. Incubation of peripheral blood mononuclear cells with 1 mM DACE resulted in higher intracellular concentrations of cysteine and GSH after 24 h than incubations with equimolar concentrations of cysteine,N-acetylcysteine, or oxothiazolidine carboxylic acid, respectively. It is concluded that DACE provides an efficient delivery system for cysteine that markedly increases intra- and extracellular cysteine and GSH after i.v. and i.d. administrations. Because its uptake into cells is probably not dependent on an active transport process, DACE results in higher intracellular concentrations of cysteine than those resulting from other prodrugs of cysteine and cysteine itself. The compound may thus have advantages over other compounds for the correction of a deranged sulfhydryl status.

Footnotes

  • Send reprint requests to: Dr. Ignazio Grattagliano, M.D., Dipartimento di Medicina Interna e Pubblica (DIMIMP), Piazza G. Cesare, 11-70124 Bari, Italy. E-mail:igratta{at}tin.it

  • ↵1 This work was supported by Grant 32-29943.90 from the Swiss National Foundation for Scientific Research.

  • Abbreviations:
    NAC
    N-acetyl-l-cysteine
    DACE
    S,N-diacetylcysteine monoethyl ester
    GSH
    glutathione
    OTC
    2-oxothiazolidine-4-carboxylate
    PBMC
    peripheral blood mononuclear cells
    i.d.
    intraduodenal
    AUC
    area under the concentration time curve
    • Received November 3, 1999.
    • Accepted March 16, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 294 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 294, Issue 1
1 Jul 2000
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Research ArticleTOXICOLOGY

Effect of Oral and IntravenousS,N-Diacetylcysteine Monoethyl Ester on Circulating and Hepatic Sulfhydryls in the Rat

Ignazio Grattagliano, Gianluigi Vendemiale, Veronika B. Deiss, Edith Junker and Bernhard H. Lauterburg
Journal of Pharmacology and Experimental Therapeutics July 1, 2000, 294 (1) 155-159;

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Research ArticleTOXICOLOGY

Effect of Oral and IntravenousS,N-Diacetylcysteine Monoethyl Ester on Circulating and Hepatic Sulfhydryls in the Rat

Ignazio Grattagliano, Gianluigi Vendemiale, Veronika B. Deiss, Edith Junker and Bernhard H. Lauterburg
Journal of Pharmacology and Experimental Therapeutics July 1, 2000, 294 (1) 155-159;
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