Abstract
Both solid- and liquid-phase combinatorial chemistry have emerged as powerful tools for identifying pharmacologically active compounds and optimizing the biological activity of a lead compound. Complementary high-throughput in vitro assays are essential for compound evaluation. Cell-based assays that use optical endpoints permit investigation of a wide variety of functional properties of these compounds including specific intracellular biochemical pathways, protein-protein interactions, and the subcellular localization of targets. Integration of combinatorial chemistry with contemporary pharmacology now represents an important factor in drug discovery and development.
Footnotes
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Send reprint requests to: John S. Lazo, Department of Pharmacology, Biomedical Science Tower E-1340, University of Pittsburgh, Pittsburgh, PA 15261. E-mail:lazo+{at}pitt.edu
- Abbreviations:
- SPOS
- solid-phase organic synthesis
- FRET
- fluorescence resonance energy transfer
- Received December 6, 1999.
- Accepted February 7, 2000.
- The American Society for Pharmacology and Experimental Therapeutics
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