Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleCELLULAR AND MOLECULAR

Neurokinin3 Receptors Couple to the Activation of Neuronal Nitric-Oxide Synthase in Stably Transfected Chinese Hamster Ovary Cells

David R. Linden, Melissa J. Chell, Esam E. El-Fakahany and Virginia S. Seybold
Journal of Pharmacology and Experimental Therapeutics May 2000, 293 (2) 559-568;
David R. Linden
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Melissa J. Chell
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Esam E. El-Fakahany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Virginia S. Seybold
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Several physiological effects induced by activation of neurokinin3 (NK3) receptors are mediated by the production of nitric oxide (NO). We investigated the intracellular coupling of NK3 receptors to NO synthase (NOS) using a Chinese hamster ovary cell line that was stably transfected with both the NK3 receptor and type I (neuronal) NOS. NOS activity in the transfected cell line was assayed directly, by measuring the formation of l-citrulline, another product of NOS, as well as indirectly, by measuring the production of cGMP in cultured rat fetal lung fibroblasts (RFL-6 cells). MePhe7-neurokinin B (NKB) stimulation of l-[3H]citrulline production was concentration-dependent and yielded a two-site model for the concentration-response relationship. The production ofl-citrulline in response to two other tachykinins, substance P or neurokinin A, revealed only a one-site nature of the response. The production of cGMP in response to MePhe7-NKB had an EC50 value that corresponded to the high-potency component of MePhe7-NKB-induced production ofl-[3H]citrulline. Agonist-induced calcium signaling was also concentration-dependent, and the acute increase in the production of cGMP by MePhe7-NKB (0.1 nM) was dependent on the release of calcium from intracellular stores. Results of this study provide the first direct evidence that NK3 receptors couple to the generation of NO within the same cell.

Footnotes

  • Send reprint requests to: Dr. Virginia S. Seybold, Department of Neuroscience, 6-145 Jackson Hall, 321 Church St. S.E., Minneapolis, MN 55455. E-mail: ginger{at}med.umn.edu

  • ↵1 This work was supported by grants from the National Institute of Neurological Disorders and Stroke, National Institutes of Health (Grants NS17702 to V.S.S. and NS25743 to E.E.E.). D.R.L. was supported by a grant from the National Institute on Drug Abuse, National Institutes of Health (Grant T32-DA07234).

  • Abbreviations:
    NK
    neurokinin
    NO
    nitric oxide
    nNOS
    neuronal NO synthase
    CHO
    Chinese hamster ovary
    RFL
    rat fetal lung
    IP3
    inositol-1,4,5-trisphosphate
    BAPTA-AM
    1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
    cPTIO
    2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
    PKC
    protein kinase C
    • Received October 26, 1999.
    • Accepted January 17, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 293 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 293, Issue 2
1 May 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Neurokinin3 Receptors Couple to the Activation of Neuronal Nitric-Oxide Synthase in Stably Transfected Chinese Hamster Ovary Cells
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleCELLULAR AND MOLECULAR

Neurokinin3 Receptors Couple to the Activation of Neuronal Nitric-Oxide Synthase in Stably Transfected Chinese Hamster Ovary Cells

David R. Linden, Melissa J. Chell, Esam E. El-Fakahany and Virginia S. Seybold
Journal of Pharmacology and Experimental Therapeutics May 1, 2000, 293 (2) 559-568;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleCELLULAR AND MOLECULAR

Neurokinin3 Receptors Couple to the Activation of Neuronal Nitric-Oxide Synthase in Stably Transfected Chinese Hamster Ovary Cells

David R. Linden, Melissa J. Chell, Esam E. El-Fakahany and Virginia S. Seybold
Journal of Pharmacology and Experimental Therapeutics May 1, 2000, 293 (2) 559-568;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Experimental Procedures
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • CsA downregulates Selenop expression via a STAT3-FoxO1 axis
  • Anisodamine Ameliorates Acute Lung Injury
  • ACE2 Inhibits LPS-Caused Lung Fibrosis
Show more Cellular and Molecular

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics