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Research ArticleNEUROPHARMACOLOGY

Coexpression of Full-Length γ-Aminobutyric AcidB(GABAB) Receptors with Truncated Receptors and Metabotropic Glutamate Receptor 4 Supports the GABAB Heterodimer as the Functional Receptor

Richard Sullivan, Anne Chateauneuf, Nathalie Coulombe, Lee F. Kolakowski Jr., Michael P. Johnson, Terence E. Hebert, Nathalie Ethier, Michel Belley, Kathleen Metters, Mark Abramovitz, Gary P. O'Neill and Gordon Y. K. Ng
Journal of Pharmacology and Experimental Therapeutics May 2000, 293 (2) 460-467;
Richard Sullivan
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Anne Chateauneuf
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Nathalie Coulombe
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Lee F. Kolakowski Jr.
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Michael P. Johnson
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Terence E. Hebert
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Nathalie Ethier
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Michel Belley
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Kathleen Metters
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Mark Abramovitz
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Gary P. O'Neill
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Gordon Y. K. Ng
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Abstract

Direct evidence is lacking to show whether the γ-aminobutyric acid (GABA)B gb1-gb2 heterodimer is the signaling form of the receptor. In this study, we tested whether gb1a or gb2 subunits when coexpressed with truncated receptors or metabotropic glutamate receptor mGluR4 could form functional GABA receptors. Coexpression of the ligand binding N-terminal domain of gb1a or the C-terminal portion of gb1a composing the seven-transmembrane segments and intracellular loops with gb2 could not reconstitute functional receptors. We next examined whether mGluR4, which forms homodimers and is structurally related to GABAB, could act as a surrogate coreceptor for gb1 or gb2. The coexpression of mGluR4 and gb1a led to the expression of gb1a monomers on cell surface membranes as determined by immunoblot analysis and flow cytometry. However, mGluR4-gb1a heterodimers were not formed, and membrane-expressed gb1a monomers were not functionally coupled to adenylyl cyclase in human embryonic kidney 293 cells or activated inwardly rectifying potassium (Kir) channels in Xenopusoocytes. Similarly, the coexpression of mGluR4 and gb2 led to nonfunctional GABA receptors. GABA-activated distal signaling events resulted only after the coexpression and heterodimerization of gb1 and gb2. Taken together with the truncated receptor studies, the data suggest that a high degree of structural specificity is required to form the functional GABAB receptor that is a gb1-gb2 heterodimer.

Footnotes

  • Send reprint requests to: Dr. Gordon Y. K. Ng, Departments of Biochemistry, Molecular Biology and Chemistry, Merck Frosst Center for Therapeutic Research, 16711 TransCanada Hwy., Kirkland, Quebec, H9H 3L1 Canada. E-mail: gordon_ng{at}merck.com

  • ↵1 The work in the laboratory of T.E.H. was supported by the Medical Research Council of Canada, the Heart and Stroke Foundation of Canada, and the Fonds de la Recherche en Santé du Québec.

  • Abbreviations:
    GABA
    γ-aminobutyric acid
    mGluR
    metabotropic glutamate receptor
    GPCR
    G protein-coupled receptor
    TM
    transmembrane domain
    Kir
    inwardly rectifying potassium channel
    HEK
    human embryonic kidney, PCR, polymerase chain reaction
    bp
    base pair(s)
    • Received November 12, 1999.
    • Accepted January 27, 2000.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 293 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 293, Issue 2
1 May 2000
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Research ArticleNEUROPHARMACOLOGY

Coexpression of Full-Length γ-Aminobutyric AcidB(GABAB) Receptors with Truncated Receptors and Metabotropic Glutamate Receptor 4 Supports the GABAB Heterodimer as the Functional Receptor

Richard Sullivan, Anne Chateauneuf, Nathalie Coulombe, Lee F. Kolakowski, Michael P. Johnson, Terence E. Hebert, Nathalie Ethier, Michel Belley, Kathleen Metters, Mark Abramovitz, Gary P. O'Neill and Gordon Y. K. Ng
Journal of Pharmacology and Experimental Therapeutics May 1, 2000, 293 (2) 460-467;

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Research ArticleNEUROPHARMACOLOGY

Coexpression of Full-Length γ-Aminobutyric AcidB(GABAB) Receptors with Truncated Receptors and Metabotropic Glutamate Receptor 4 Supports the GABAB Heterodimer as the Functional Receptor

Richard Sullivan, Anne Chateauneuf, Nathalie Coulombe, Lee F. Kolakowski, Michael P. Johnson, Terence E. Hebert, Nathalie Ethier, Michel Belley, Kathleen Metters, Mark Abramovitz, Gary P. O'Neill and Gordon Y. K. Ng
Journal of Pharmacology and Experimental Therapeutics May 1, 2000, 293 (2) 460-467;
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