Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleCELLULAR AND MOLECULAR

Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells

Chung-Ren Jan, Lee-Wei Chen and Ming-Wei Lin
Journal of Pharmacology and Experimental Therapeutics March 2000, 292 (3) 995-1001;
Chung-Ren Jan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lee-Wei Chen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ming-Wei Lin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The effect of chloroform on Ca2+ mobilization in Madin-Darby canine kidney cells was examined by using Fura-2 as a Ca2+ probe. Chloroform (24–248 mM) concentration dependently increased intracellular Ca2+ concentration ([Ca2+]i). Ca2+ removal inhibited the Ca2+ signals evoked by 93 to 248 mM chloroform by reducing both the initial rise and the sustained phase. In Ca2+-free medium, pretreatment with 93 mM chloroform abolished the Ca2+ release induced by 1 μM thapsigargin, an endoplasmic reticulum Ca2+ pump inhibitor, and partially reduced the Ca2+ release induced by 2 μM carbonylcyanidem-chlorophenylhydrazone, a mitochondrial uncoupler. Pretreatment with carbonylcyanidem-chlorophenylhydrazone and thapsigargin to deplete the Ca2+ stores in mitochondria and the endoplasmic reticulum, respectively, only partially inhibited chloroform-induced Ca2+ release. This suggests that chloroform released Ca2+ from multiple internal pools. The addition of 3 mM Ca2+ increased [Ca2+]i after pretreatment with 93 mM chloroform in Ca2+-free medium. La3+ (1 mM) partially inhibited the [Ca2+]i increase induced by 93 mM chloroform. Chloroform (93 mM)-induced Ca2+ release was not altered when the formation of inositol-1,4,5-trisphosphate was abolished by U73122 (2 μM), a phospholipase C inhibitor, but was inhibited by 90% by inhibition of phospholipase A2 with 40 μM aristolochic acid. Collectively, we found that 93 mM chloroform increased [Ca2+]i in Madin-Darby canine kidney cells by releasing Ca2+ from multiple stores in a manner independent of the formation of inositol-1,4,5-trisphosphate, followed by Ca2+ entry from external medium. Other solvents, such as ethanol, methanol, and DMSO, did not affect the resting [Ca2+]i at a concentration of 248 mM.

Footnotes

  • Send reprint requests to: C. R. Jan, Ph.D., Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, 386 Ta Chung 1st Rd., Kaohsiung, Taiwan 813. E-mail:crjan{at}isca.vghks.gov.tw

  • ↵1 This work was supported by grants from National Science Council (NSC89-2320-B-075B-009), VTY Joint Research Program, Tsou's Foundation (VTY88-P3-24), and Veterans General Hospital-Kaohsiung (VGHKS89-13) to C.-R.J.

  • Abbreviations:
    [Ca2+]i
    intracellular Ca2+ concentration
    IP3
    inositol-1,4,5-trisphosphate
    MDCK
    Madin-Darby canine kidney
    CCCP
    carbonylcyanide m-chlorophenylhydrazone
    • Received September 23, 1999.
    • Accepted December 2, 1999.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 292 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 292, Issue 3
1 Mar 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleCELLULAR AND MOLECULAR

Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells

Chung-Ren Jan, Lee-Wei Chen and Ming-Wei Lin
Journal of Pharmacology and Experimental Therapeutics March 1, 2000, 292 (3) 995-1001;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleCELLULAR AND MOLECULAR

Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells

Chung-Ren Jan, Lee-Wei Chen and Ming-Wei Lin
Journal of Pharmacology and Experimental Therapeutics March 1, 2000, 292 (3) 995-1001;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Chlorogenic Acid Inhibits Breast Cancer Metastasis
  • SNAP25 and mGluRs Control Pathological Tau Release
  • N-Stearoylethanolamine Inhibits Platelet Reactivity
Show more Cellular and Molecular

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics