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Research ArticleCARDIOVASCULAR

Cytochrome P450 ω/ω-1 Hydroxylase-Derived Eicosanoids Contribute to EndothelinA and EndothelinBReceptor-Mediated Vasoconstriction to Endothelin-1 in the Rat Preglomerular Arteriole

Hantz C. Hercule and Adebayo O. Oyekan
Journal of Pharmacology and Experimental Therapeutics March 2000, 292 (3) 1153-1160;
Hantz C. Hercule
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Adebayo O. Oyekan
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Abstract

The preglomerular arteriole of the rat was used to evaluate the contribution of cytochrome P450-derived eicosanoids to the vasoconstrictor effect of endothelin (ET)-1 and to determine the receptors mediating the response. ET-1 (4 × 10−11 to 2 × 10−9 M) produced dose-dependent reductions in the intraluminal diameter of the renal arteriole ranging from 25 ± 8 to 142 ± 16 μm. BMS182874 [(5-dimethylamino)-N-(3,4-dimethyl-5-isoxazolyl)-1-naphthalenesulfonamide; 3 μM], an ETA receptor antagonist, or BQ788 (N-cis-2,6-dimethyl-piperidino-carbonyl-l-γ-methylleucyl-d-1-methoxycarbonyl-tryptophanyl-d-norleucine; 1 μM), an ETB receptor antagonist, attenuated ET-1 vasoconstriction by 59 ± 4 and 50 ± 10%, respectively. The combined administration of both ET receptor antagonists increased inhibition of ET-1 vasoconstriction to 75 ± 4%. 17-Octadecynoic acid (17-ODYA, 2 μM) or 12,12-dibromododec-enoic acid (2 μM), inhibitors of 20-hydroxyeicosatetraenoic acid (20-HETE) production, attenuated ET-1-induced vasoconstriction by 50 ± 6 and 40 ± 3%, respectively, as did indomethacin (10 μM), an inhibitor of cyclooxygenase. Miconazole (2 μM), the epoxygenase inhibitor, was without effect. 20-HETE (10−8 and 2 × 10−8 M) elicited a dose-related vasoconstriction that was inhibited by 10 μM, but not 5 μM, indomethacin. The inhibition by 17-ODYA of ET-1 vasoconstriction was not greater when combined with BMS182874 or BQ788. Moreover, vasoconstriction induced by ET-3, an ETB-selective agonist, was inhibited by 17-ODYA. These data indicate that both ETA and ETB receptors mediate ET-1 vasoconstriction and that 20-HETE production linked to both receptors makes a major contribution to ET-1-induced renal arteriolar vasoconstriction in the rat.

Footnotes

  • Send reprint requests to: Dr. A. O. Oyekan, Center for Cardiovascular Diseases, College of Pharmacy and Health Sciences, Texas Southern University, 3100 Cleburne Ave., Houston, TX 77004. E-mail: oyekan_ao{at}tsu.edu

  • ↵1 This work was supported by National Institutes of Health Grant RO1-HL25394 and RO1-HL59884. This study was presented at the Experimental Biology '99 Meeting in Washington, DC, April 7–21, 1999.

  • Abbreviations:
    ET
    endothelin
    Ang II
    angiotensin II
    ID
    intraluminal diameter
    COX
    cyclooxygenase
    AA
    arachidonic acid
    LOX
    lipoxygenase
    MOX
    monooxygenase
    CYP
    cytochrome P450
    17-ODYA
    17-octadecynoic acid
    DBDD
    12,12-dibromododec-enoic acid
    ETYA
    5,8,11,14-eicosatetraynoic acid
    PE
    phenylephrine
    • Received July 15, 1999.
    • Accepted November 10, 1999.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 292 (3)
Journal of Pharmacology and Experimental Therapeutics
Vol. 292, Issue 3
1 Mar 2000
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Research ArticleCARDIOVASCULAR

Cytochrome P450 ω/ω-1 Hydroxylase-Derived Eicosanoids Contribute to EndothelinA and EndothelinBReceptor-Mediated Vasoconstriction to Endothelin-1 in the Rat Preglomerular Arteriole

Hantz C. Hercule and Adebayo O. Oyekan
Journal of Pharmacology and Experimental Therapeutics March 1, 2000, 292 (3) 1153-1160;

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Research ArticleCARDIOVASCULAR

Cytochrome P450 ω/ω-1 Hydroxylase-Derived Eicosanoids Contribute to EndothelinA and EndothelinBReceptor-Mediated Vasoconstriction to Endothelin-1 in the Rat Preglomerular Arteriole

Hantz C. Hercule and Adebayo O. Oyekan
Journal of Pharmacology and Experimental Therapeutics March 1, 2000, 292 (3) 1153-1160;
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