Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Mechanism of Aloe Single Component (Alprogen) on Mediator Release in Guinea Pig Lung Mast Cells Activated with Specific Antigen-Antibody Reactions

Jai Youl Ro, Byung Chul Lee, Ji Young Kim, Yean Jun Chung, Myung Hee Chung, Seung Kee Lee, Tae Hyung Jo, Kyung Hwan Kim and Young In Park
Journal of Pharmacology and Experimental Therapeutics January 2000, 292 (1) 114-121;
Jai Youl Ro
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Byung Chul Lee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ji Young Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yean Jun Chung
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Myung Hee Chung
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seung Kee Lee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tae Hyung Jo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kyung Hwan Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Young In Park
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

We previously reported that the glycoprotein extracted from aloe strongly inhibited the mediator releases caused by the activation of guinea pig lung mast cells. Therefore, this study aimed to purify a single component that has an antiallergic effect from crude aloe extract and then to assess the effects of aloe single component (alprogen) on the mechanism of mediator releases caused by the mast cell activation. We purified aloe extracts by using various columns. We also purified mast cells from guinea pig lung tissues by using enzyme digestion, rough and discontinuous density Percoll gradient. Mast cells were sensitized with IgG1(anti-ovalbumin) and challenged with ovalbumin. Histamine was assayed by using a fluorometric analyzer and leukotrienes by radioimmunoassay. [Ca2+]i level was analyzed by using a confocal laser scanning microscope. Protein kinase activity was determined by the protein phosphorylated with [γ-32P]ATP. The phospholipase D activity was assessed by the labeled phosphatidylalcohol. The amount of mass 1,2-diacylglycerol (DAG) was measured by the [3H]DAG produced when prelabeled with [3H]myristic acid. Phospholipase A2 activity was determined by measuring the lyso-phosphatidylcholine released from the labeled phospholipids. Alprogen significantly decreased histamine and leukotriene releases and blocked completely Ca2+ influx during mast cell activation. The protein kinase C and phospholipase D activities were decreased by alprogen in dose-dependent manner. Alprogen inhibited mass DAG formation and the phospholipase A2 activity during mast cell activation. The data suggest that alprogen purified from aloe inhibits multiple signals as well as blocking Ca2+ influx caused by mast cells activated with specific antigen-antibody reactions and that then the inhibition of histamine and leukotriene release follows.

Footnotes

  • Send reprint requests to: Dr. Jai Youl Ro, Department of Pharmacology, Yonsei University College of Medicine, CPO Box 8044, Seoul, Korea, 120-752. E-mail:JYRO426{at}yumc.yonsei.ac.kr

  • ↵1 This study was supported by the 1995–1998 STEPI (Science and Technology Policy Institute) Grant and a Nam Yang Institute Grant in Seoul, Korea.

  • Abbreviations:
    PLD
    phospholipase D
    OA
    ovalbumin
    PBut
    phosphatidylbutanol
    DAG
    1,2-diacylglycerol
    PKC
    protein kinase C
    PLA2
    phospholipase A2
    PC
    phosphatidylcholine
    PAPC
    1-palmitoyl-2-arachidonyl phosphatidyl-[14C]choline
    S.A.
    specific activity
    • Received February 5, 1999.
    • Accepted August 16, 1999.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics: 292 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 292, Issue 1
1 Jan 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Inhibitory Mechanism of Aloe Single Component (Alprogen) on Mediator Release in Guinea Pig Lung Mast Cells Activated with Specific Antigen-Antibody Reactions
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Mechanism of Aloe Single Component (Alprogen) on Mediator Release in Guinea Pig Lung Mast Cells Activated with Specific Antigen-Antibody Reactions

Jai Youl Ro, Byung Chul Lee, Ji Young Kim, Yean Jun Chung, Myung Hee Chung, Seung Kee Lee, Tae Hyung Jo, Kyung Hwan Kim and Young In Park
Journal of Pharmacology and Experimental Therapeutics January 1, 2000, 292 (1) 114-121;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleGASTROINTESTINAL, HEPATIC, PULMONARY, AND RENAL

Inhibitory Mechanism of Aloe Single Component (Alprogen) on Mediator Release in Guinea Pig Lung Mast Cells Activated with Specific Antigen-Antibody Reactions

Jai Youl Ro, Byung Chul Lee, Ji Young Kim, Yean Jun Chung, Myung Hee Chung, Seung Kee Lee, Tae Hyung Jo, Kyung Hwan Kim and Young In Park
Journal of Pharmacology and Experimental Therapeutics January 1, 2000, 292 (1) 114-121;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • H2S Overproduction and Colonic Hypomotility in DM
  • A Novel Long-Acting GLP-2, HM15912, for Short Bowel Syndrome
  • MIP3a in Progressive Renal Injury Associated With Obesity
Show more Gastrointestinal, Hepatic, Pulmonary, and Renal

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics