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Research ArticleArticle

Nicotine Infusion Modulates Immobilization Stress-Triggered Induction of Gene Expression of Rat Catecholamine Biosynthetic Enzymes

Lidia Serova, Emil Danailov, Firas Chamas and Esther L. Sabban
Journal of Pharmacology and Experimental Therapeutics November 1999, 291 (2) 884-892;
Lidia Serova
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Emil Danailov
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Firas Chamas
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Esther L. Sabban
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Abstract

The relationship between nicotine and stress is complex and paradoxical. Although people claim they smoke because it relaxes them, nicotine can trigger some of the effects observed with stress, including the release and synthesis of the catecholamines and their biosynthetic enzymes. This study examined one aspect of this confusing relationship between nicotine and stress. Multiple injections of nicotine bitartrate (5 mg/kg) elevated mRNA levels for the catecholamine biosynthetic enzymes, tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), and phenylethanolamineN-methyltransferase, and of preproneuropeptide Y in rat adrenal medulla more than did 1 mg/kg of nicotine bitartrate. In the locus ceruleus, substantia nigra, and ventral tegmental area both doses equally induced TH mRNA levels. Nicotine infusion (15 mg/kg/day) did not affect adrenal mRNA levels for any of the genes of interest and did not increase plasma corticosterone levels. However, in rats pre-exposed to nicotinic infusions, the response to a single immobilization (IMO) stress was markedly attenuated with respect to changes in adrenomedullary TH, DBH, and phenylethanolamineN-methyltransferase mRNA levels and in c-Fos protein levels. In the central nervous system, the chronic infusion of nicotine prevented the induction of TH mRNA by repeated IMO stress in the ventral tegmental area (but not in substantia nigra) and of DBH mRNA by single IMO in the locus ceruleus. These findings may explain some of the complex interactions between stress and exposure to nicotine.

Footnotes

  • Send reprint requests to: Esther L. Sabban, Ph.D., Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595. E-mail:Sabban{at}nymc.edu

  • ↵1 Supported by Grant NS32166 (to E.L.S.) from the National Institutes of Health and Grant 0251 from the Smokeless Tobacco Research Council.

  • Abbreviations:
    TH
    tyrosine hydroxylase
    DBH
    dopamine β-hydroxylase
    IMO
    immobilization stress
    LC
    locus ceruleus
    NPY
    neuropeptide Y
    PNMT
    phenylethanolamineN-methyltransferase
    SN
    substantia nigra
    VTA
    ventral tegmental area
    CNS
    central nervous system
    • Received January 25, 1999.
    • Accepted July 20, 1999.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 291 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 291, Issue 2
1 Nov 1999
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Research ArticleArticle

Nicotine Infusion Modulates Immobilization Stress-Triggered Induction of Gene Expression of Rat Catecholamine Biosynthetic Enzymes

Lidia Serova, Emil Danailov, Firas Chamas and Esther L. Sabban
Journal of Pharmacology and Experimental Therapeutics November 1, 1999, 291 (2) 884-892;

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Research ArticleArticle

Nicotine Infusion Modulates Immobilization Stress-Triggered Induction of Gene Expression of Rat Catecholamine Biosynthetic Enzymes

Lidia Serova, Emil Danailov, Firas Chamas and Esther L. Sabban
Journal of Pharmacology and Experimental Therapeutics November 1, 1999, 291 (2) 884-892;
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