Abstract
The biochemical influence of flavonolignans from the milk thistleSilybum marianum has been tested on kidney cells of African green monkeys. Two nonmalignant cell lines were selected, with the focus of the work on the fibroblast-like Vero line. Proliferation rate, biosynthesis of protein and DNA, and the activity of the enzyme lactate dehydrogenase (as a measure of the cellular metabolic activity) were chosen as parameters for the effect of the flavonolignans. Silibinin and silicristin show remarkable stimulatory effects on these parameters, mainly in Vero cells; however, isosilibinin and silidianin proved to be inactive. In vitro experiments with kidney cells damaged by paracetamol, cisplatin, and vincristin demonstrated that administration of silibinin before or after the chemical-induced injury can lessen or avoid the nephrotoxic effects. The results warrant in vivo evaluations of the flavonolignan derivatives.
Footnotes
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Send reprint requests to: Dr. Johann Sonnenbichler, Max Planck Institut für Biochemie, Am Klopferspitz 21, D-82152 Martinsried, Germany.
- Abbreviations:
- TCA
- trichloroacetic acid
- LDH
- lactate dehydrogenase
- NADH
- nicotine adeninedinucleotide
- Received September 2, 1998.
- Accepted May 25, 1999.
- The American Society for Pharmacology and Experimental Therapeutics
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