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Research ArticleArticle

Combination of Hypoxia/Aglycemia Compromises In Vitro Blood-Brain Barrier Integrity

Thomas J. Abbruscato and Thomas P. Davis
Journal of Pharmacology and Experimental Therapeutics May 1999, 289 (2) 668-675;
Thomas J. Abbruscato
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Thomas P. Davis
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Abstract

Increased cerebrovascular permeability is an important factor in the development of cerebral edema after stroke, implicating the blood-brain barrier (BBB) in the pathology of stroke. Present investigations modeled stroke at the level of the cerebral capillary endothelium by analyzing BBB permeability changes to the membrane-impermeant marker [14C]sucrose after hypoxia/aglycemia. Under hypoxia alone, long exposures (48 h) were necessary to result in a significant increase in permeability of bovine brain microvessel endothelial cells to [14C]sucrose. Hypoxia/aglycemia exposures resulted in a much shorter time (i.e., 1–3 h) required for a corresponding increase in permeability to [14C]sucrose. Statistically significant changes in basal permeability were observed between 3 and 6 h of hypoxia/aglycemia; however, 6 h of aglycemia alone had no significant effect on BBB permeability. Both rat astroglioma (C6) cells and C6 conditioned medium showed no improvements in barrier function measured by transendothelial cell resistance or permeability to [14C]sucrose. Changes in endothelial cell calcium flux may be responsible for the permeability change observed after both 48 h of hypoxia and 6 h of hypoxia/aglycemia because nifedipine (10 and 100 nM) and SKF 96365 (100 nM) decreased the permeability change. Immunocytochemical studies also revealed a change in the distribution of endothelial cell F-actin. This study provides evidence that the BBB is sensitive to short exposures of hypoxia/aglycemia and that changes in endothelial cell calcium flux may be responsible for structural and functional variations in the BBB during ischemic stress.

Footnotes

  • Send reprint requests to: Thomas P. Davis, Ph.D., Department of Pharmacology, University of Arizona College of Medicine, 1501 N. Campbell Ave., Tucson, AZ 85724. E-mail: davistp{at}u.arizona.edu

  • ↵1 This work was supported by U.S. Public Health Services Grant NIH RO1-DA11271 (T.P.D.) and an NRSA fellowship from NINDS (NS10580) to (T.J.A.).

  • Abbreviations:
    BBB
    blood-brain barrier
    CNS
    central nervous system
    BBMEC
    bovine brain microvessel endothelial cell
    PC
    permeability coefficient
    C6
    rat astroglioma
    CM
    conditioned medium
    TEER
    transendothelial cell resistance
    DPDPE
    [d-Pen2,d-Pen5]-enkephalin
    FITC
    fluorescein isothiocyanate
    • Received October 14, 1998.
    • Accepted December 14, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 289 (2)
Journal of Pharmacology and Experimental Therapeutics
Vol. 289, Issue 2
1 May 1999
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Research ArticleArticle

Combination of Hypoxia/Aglycemia Compromises In Vitro Blood-Brain Barrier Integrity

Thomas J. Abbruscato and Thomas P. Davis
Journal of Pharmacology and Experimental Therapeutics May 1, 1999, 289 (2) 668-675;

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Research ArticleArticle

Combination of Hypoxia/Aglycemia Compromises In Vitro Blood-Brain Barrier Integrity

Thomas J. Abbruscato and Thomas P. Davis
Journal of Pharmacology and Experimental Therapeutics May 1, 1999, 289 (2) 668-675;
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