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Research ArticleArticle

Inhibitory Effect of Rebamipide on the Neutrophil Adherence Stimulated by Conditioned Media from Helicobacter pylori-Infected Gastric Epithelial Cells

Chi Dae Kim, Hyun Hee Kim and Ki Whan Hong
Journal of Pharmacology and Experimental Therapeutics January 1999, 288 (1) 133-138;
Chi Dae Kim
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Hyun Hee Kim
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Ki Whan Hong
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Abstract

We investigated the mechanism or mechanisms by which rebamipide protects against the gastric mucosal inflammation associated withHelicobacter pylori. The production of interleukin (IL)-8 in association with expression of IL-8 mRNA was greatly increased in the H. pylori-infected Kato III cells in a concentration- and time-dependent manner, whereas the secretion of IL-6 and tumor necrosis factor-α was not detectable. The increased production of IL-8 and expression of IL-8 mRNA were significantly inhibited by rebamipide (100–1000 μM) in a concentration-dependent manner. Formyl-methionyl-leucyl-phenylalanine (1 nM), as well as conditioned medium (CM) that was produced from H. pylori-infected Kato III cells, caused an increase in surface expression of CD11b on human neutrophils and an increase in neutrophil adhesion to the human umbilical vein endothelial cells. Rebamipide also suppressed the adherence of neutrophils to endothelial cells as well as the expression of CD11b on neutrophils induced by formyl-methionyl-leucyl-phenylalanine and CM. Furthermore, CM-induced neutrophil adhesion to the endothelial cells was significantly inhibited by IL-8-neutralizing antibody, suggesting that IL-8 is implicated in the CM-induced neutrophil adhesion to the cultured human umbilical vein endothelial cells. It is concluded that rebamipide exerts its preventive effect against H. pylori-evoked gastric mucosal cell inflammation by inhibition of the neutrophil adherence to the endothelial cells as well as by suppressing the surface expression of CD11b on neutrophils and the production of proinflammatory cytokine such as IL-8 from gastric epithelial cells.

Footnotes

  • Send reprint requests to: Ki Whan Hong, M.D., Doctor of Philosophy Department of Pharmacology, College of Medicine, Pusan National University, Ami-Dong 1-Ga, Seo-Gu Pusan 602-739, Korea. E-mail: kwhong{at}hyowon.pusan.ac.kr

  • ↵1 This study was supported by research funds from Korea Otsuka Pharmaceutical Co., Ltd., and the Center for Biofunctional Molecules at Pohang University of Science and Technology.

  • Abbreviations:
    IL
    interleukin
    fMLP
    formyl-methionyl-leucyl-phenylalanine
    HUVEC
    human umbilical vein endothelial cell
    CM
    conditioned medium
    RT
    reverse transcription
    PCR
    polymerase chain reaction
    TNF
    tumor necrosis factor
    PBS
    phosphate-buffered saline
    • Received May 22, 1998.
    • Accepted August 6, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics: 288 (1)
Journal of Pharmacology and Experimental Therapeutics
Vol. 288, Issue 1
1 Jan 1999
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Research ArticleArticle

Inhibitory Effect of Rebamipide on the Neutrophil Adherence Stimulated by Conditioned Media from Helicobacter pylori-Infected Gastric Epithelial Cells

Chi Dae Kim, Hyun Hee Kim and Ki Whan Hong
Journal of Pharmacology and Experimental Therapeutics January 1, 1999, 288 (1) 133-138;

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Research ArticleArticle

Inhibitory Effect of Rebamipide on the Neutrophil Adherence Stimulated by Conditioned Media from Helicobacter pylori-Infected Gastric Epithelial Cells

Chi Dae Kim, Hyun Hee Kim and Ki Whan Hong
Journal of Pharmacology and Experimental Therapeutics January 1, 1999, 288 (1) 133-138;
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