Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleArticle

Participation of Nitric Oxide in the Mucosal Injury of Rat Intestine Induced by Ischemia-Reperfusion

Kohei Takada, Kimihiro Yamashita, Yasuko Sakurai-Yamashita, Kazuto Shigematsu, Yoshinori Hamada, Kohshiro Hioki and Kohtaro Taniyama
Journal of Pharmacology and Experimental Therapeutics October 1998, 287 (1) 403-407;
Kohei Takada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kimihiro Yamashita
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yasuko Sakurai-Yamashita
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuto Shigematsu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoshinori Hamada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kohshiro Hioki
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kohtaro Taniyama
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The dual role of nitric oxide as a cytoprotective or a cytotoxic free radical gas has been noted in various types of pathophysiological conditions, including the digestive system. The aim of this study was to examine the role of nitric oxide in the mucosal injury induced by ischemia-reperfusion in the rat small intestine. A transient intestinal ischemia was produced in the catheterized ileal segments of rats by occluding the anterior mesenteric artery for 60 min. Nitric oxide metabolites (NO2− and NO3−) and lactate dehydrogenase activity in perfusates of the intestinal lumen were measured over 5 hr periods. The time-course of histological changes in small intestine was also observed. After ischemia-reperfusion, nitric oxide release in the intestinal lumen increased significantly and the dynamics of nitric oxide release correlated with that of lactate dehydrogenase leakage. The administration of NG-nitro-l-arginine methyl ester (1.0–2.5 mg/kg) inhibited this increased nitric oxide release and the lactate dehydrogenase leakage and afforded protection against the mucosal injury induced by ischemia-reperfusion. In conclusion, the nitric oxide production that was accelerated by ischemia-reperfusion of small intestine may possibly participate in the breakdown of intestinal mucosa after ischemia-reperfusion insult.

Footnotes

  • Send reprint requests to: Kimihiro Yamashita, Ph.D., Department of Pharmacology, Nagasaki University School of Medicine, 1–12-4 Sakamoto, Nagasaki 852-8523, Japan.

  • ↵1 This work was supported in part by the Japan Research Foundation for Clinical Pharmacology and Grant-in-Aids for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

  • ↵2 Current address: Kimihiro Yamashita, Faculty of Environmental Studies, Nagasaki University, 1–14 Bunkyo-machi, Nagasaki 852-8521, Japan.

  • Abbreviations:
    LDH
    lactate dehydrogenase
    L-NAME
    NG-nitro-l-arginine methyl ester
    NO
    nitric oxide
    NOS
    nitric oxide synthase
    iNOS
    inducible nitric oxide synthase
    • Received March 6, 1998.
    • Accepted June 1, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 287, Issue 1
1 Oct 1998
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Participation of Nitric Oxide in the Mucosal Injury of Rat Intestine Induced by Ischemia-Reperfusion
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Participation of Nitric Oxide in the Mucosal Injury of Rat Intestine Induced by Ischemia-Reperfusion

Kohei Takada, Kimihiro Yamashita, Yasuko Sakurai-Yamashita, Kazuto Shigematsu, Yoshinori Hamada, Kohshiro Hioki and Kohtaro Taniyama
Journal of Pharmacology and Experimental Therapeutics October 1, 1998, 287 (1) 403-407;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Participation of Nitric Oxide in the Mucosal Injury of Rat Intestine Induced by Ischemia-Reperfusion

Kohei Takada, Kimihiro Yamashita, Yasuko Sakurai-Yamashita, Kazuto Shigematsu, Yoshinori Hamada, Kohshiro Hioki and Kohtaro Taniyama
Journal of Pharmacology and Experimental Therapeutics October 1, 1998, 287 (1) 403-407;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • PST3093 Stimulates SERCA2a and Improves Cardiac Function
  • CRV431 Decreases Liver Fibrosis and Tumor Development
  • Is Hydroxylamine-Induced Cytotoxicity a Valid Marker for Hypersensitivity Reactions to Sulfamethoxazole in Human Immunodeficiency Virus-Infected Individuals?
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics