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Research ArticleArticle

A Neurotensin Receptor Antagonist Inhibits Acute Immobilization Stress-Induced Cardiac Mast Cell Degranulation, a Corticotropin-Releasing Hormone-Dependent Process

Xinzhu Pang, Nicholas Alexacos, Richard Letourneau, Dimitri Seretakis, Wei Gao, William Boucher, David E. Cochrane and Theoharis C. Theoharides
Journal of Pharmacology and Experimental Therapeutics October 1998, 287 (1) 307-314;
Xinzhu Pang
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Nicholas Alexacos
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Richard Letourneau
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Dimitri Seretakis
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Wei Gao
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William Boucher
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David E. Cochrane
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Theoharis C. Theoharides
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Abstract

Stress worsens certain disorders such as migraines or asthma, and has also been implicated in sudden myocardial arrest. It was previously shown that acute psychological stress by immobilization results in dura mast cell degranulation, an effect blocked by pretreatment with antiserum against corticotropin-releasing hormone (CRH). Moreover, CRH was recently shown to induce skin mast cell degranulation. The effect of psychological stress was investigated on rat cardiac mast cells, because their release of coronary constrictive and proinflammatory molecules contributes to myocardial ischemia and possibly arrhythmias. Immobilization of rats for 30 min induced maximal cardiac mast cell degranulation as evidenced by light and electron microscopy. This effect was inhibited by pretreatment with the “antiallergic” drug sodium cromoglycate (cromolyn), which is thought to act primarily through mast cell stabilization. Mast cell degranulation was also blocked by preincubation with antiserum against CRH and was partially inhibited by a CRH type-1 receptor selective antagonist. Sensory neuropeptides did not appear to influence this effect, but a nonpeptide neurotensin receptor antagonist blocked stress-induced cardiac mast cell degranulation. This finding supports the involvement of neuropeptide neurotensin which is present in the heart and is known to trigger mast cell degranulation. These results indicate acute stress could result in local CRH and nonpeptide neurotensin release which could contribute to myocardial pathophysiology through direct or indirect release of cardiac mast cell mediators.

Footnotes

  • Send reprint requests to: Dr. T. C. Theoharides, Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111.

  • ↵1 This work was supported by a grant from Kos Pharmaceuticals, Inc., Miami, FL.

  • Abbreviations:
    CTMC
    connective tissue mast cells
    CAD
    coronary artery disease
    CRH
    corticotropin-releasing hormone
    CRHR
    CRH receptor
    cromolyn
    disodium cromoglycate
    MI
    myocardial infarction
    MMC
    mucosal mast cells
    NRS
    normal rabbit serum
    NGF
    nerve growth factor
    NT
    neurotensin
    PBS
    phosphate-buffered saline
    RMCP
    rat mast cell protease
    SP
    substance P
    • Received February 26, 1998.
    • Accepted May 26, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics
Vol. 287, Issue 1
1 Oct 1998
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Research ArticleArticle

A Neurotensin Receptor Antagonist Inhibits Acute Immobilization Stress-Induced Cardiac Mast Cell Degranulation, a Corticotropin-Releasing Hormone-Dependent Process

Xinzhu Pang, Nicholas Alexacos, Richard Letourneau, Dimitri Seretakis, Wei Gao, William Boucher, David E. Cochrane and Theoharis C. Theoharides
Journal of Pharmacology and Experimental Therapeutics October 1, 1998, 287 (1) 307-314;

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Research ArticleArticle

A Neurotensin Receptor Antagonist Inhibits Acute Immobilization Stress-Induced Cardiac Mast Cell Degranulation, a Corticotropin-Releasing Hormone-Dependent Process

Xinzhu Pang, Nicholas Alexacos, Richard Letourneau, Dimitri Seretakis, Wei Gao, William Boucher, David E. Cochrane and Theoharis C. Theoharides
Journal of Pharmacology and Experimental Therapeutics October 1, 1998, 287 (1) 307-314;
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