Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherIMMUNOPHARMACOLOGY

IL-10 Synergizes with Dexamethasone in Inhibiting Human T Cell Proliferation

Mauro Brunetti, Antonella Colasante, Nicola Mascetra, Mauro Piantelli, Piero Musiani and Francesca B. Aiello
Journal of Pharmacology and Experimental Therapeutics May 1998, 285 (2) 915-919;
Mauro Brunetti
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antonella Colasante
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicola Mascetra
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mauro Piantelli
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Piero Musiani
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Francesca B. Aiello
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

We have evaluated the effects of dexamethasone (Dex) alone or in combination with interleukin (IL)-10 or transforming growth factor-β1 (TGF-β1) on human T cell proliferation. Both IL-10 and TGF-β1 significantly decreased the Dex concentration needed to inhibit T cell proliferation by 50% (IC50). Dex in combination with IL-10 completely inhibited T cell proliferation, even when IL-10 alone was ineffective, as in the case of phytohemagglutinin-induced T cell proliferation. The evaluation of the results according to the isobole method displayed a potent synergistic activity between Dex and IL-10, whereas the combination of Dex with TGF-β1 was additive. IL-10, but not TGF-β1, enhanced the inhibitory effect of Dex on IL-2 production. IL-2 and IL-4 only partly antagonized the antiproliferative effect of the combinations. IL-4 was as effective as IL-2 in antagonizing the combination of Dex with TGF-β1, but significantly less effective against the combination of Dex with IL-10. IL-10 and TGF-β1 are thus able to potentiate the Dex inhibitory effect on T cell proliferation and could be regarded as potential agents for future immunosuppressive protocols.

Footnotes

  • Send reprint requests to: Mauro Brunetti, Laboratory of Immunopathology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy.

  • ↵1 Supported in part by grants from Ministero della Università e Ricerca Scientifica e Tecnologica (MURST) and from the “Eighth Research Project on AIDS,” Istituto Superiore di Sanità.

  • Abbreviations:
    GC
    glucocorticoids
    TGF-β1
    transforming growth factor-β1
    IL
    interleukin
    APC
    antigen-presenting cells
    Dex
    dexamethasone
    PBMC
    peripheral blood mononuclear cells
    mAb
    monoclonal antibody
    PHA
    phytohemagglutinin
    r
    recombinant
    • Received December 4, 1997.
    • Accepted January 16, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 285, Issue 2
1 May 1998
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
IL-10 Synergizes with Dexamethasone in Inhibiting Human T Cell Proliferation
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
OtherIMMUNOPHARMACOLOGY

IL-10 Synergizes with Dexamethasone in Inhibiting Human T Cell Proliferation

Mauro Brunetti, Antonella Colasante, Nicola Mascetra, Mauro Piantelli, Piero Musiani and Francesca B. Aiello
Journal of Pharmacology and Experimental Therapeutics May 1, 1998, 285 (2) 915-919;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
OtherIMMUNOPHARMACOLOGY

IL-10 Synergizes with Dexamethasone in Inhibiting Human T Cell Proliferation

Mauro Brunetti, Antonella Colasante, Nicola Mascetra, Mauro Piantelli, Piero Musiani and Francesca B. Aiello
Journal of Pharmacology and Experimental Therapeutics May 1, 1998, 285 (2) 915-919;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgment
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Modulation of Mouse Endotoxin Shock by Inhibition of Phosphatidylcholine-Specific Phospholipase C
  • Spontaneous and Cationic Lipid-Mediated Uptake of Antisense Oligonucleotides in Human Monocytes and Lymphocytes
Show more IMMUNOPHARMACOLOGY

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics