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OtherGASTROINTESTINAL PHARMACOLOGY

Colitis-Induced Changes in the Expression of the Na+/H+ Exchanger Isoform NHE-1

Islam Khan, Farida M. Al-Awadi and Habib Abul
Journal of Pharmacology and Experimental Therapeutics May 1998, 285 (2) 869-875;
Islam Khan
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Farida M. Al-Awadi
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Habib Abul
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Abstract

The sodium hydrogen exchanger (NHE) plays an important role in the absorption of NaCl, the regulation of intracellular pH and cell growth. These functions are compromised in the inflammatory bowel diseases. The objective of this study was to examine the expression of the NHE-1 isoform during colitis induced by acetic acid or trinitrobenzenesulfonic acid in Sprague-Dawley male rats. We also examined the effect of dexamethasone on the expression of NHE-1. Levels of mRNA were estimated using the reverse transcription-polymerase chain reaction and slot blot analysis, and levels of protein were estimated by enhanced chemiluminescence light Western blot analysis. The levels of the NHE-1 mRNA and protein in colonic mucosa increased as assessed at 1, 2, 5 and 7 days post-acetic acid administration and 7 days post-trinitrobenzenesulphonic acid administration in the rats. The levels of mRNA were not suppressed by dexamethasone treatment in either case. These findings demonstrate that colitis-induced expression of the NHE-1 mRNA and protein is independent of the way colitis is induced. Although factor(s) responsible for the induction remain to be identified, our findings showing similar changes in the NHE-2 and NHE-3 mRNA isoforms, together with the lack of their suppression by dexamethasone, suggest that cytokines and intracellular pH are secondary factors.

Footnotes

  • Send reprint requests to: Dr. Islam Khan, Department of Biochemistry, Faculty of Medicine, Kuwait University, P.O. Box 24923, Kuwait.

  • Abbreviations:
    HAC
    acetic acid
    TNBS
    trinitrobenzenesulfonic acid
    NHE
    sodium hydrogen exchanger
    RT-PCR
    reverse transcription-polymerase chain reaction
    ECL
    enhanced chemiluminescence light
    PBS
    phosphate-buffered saline
    SSC
    sodium chloride and sodium citrate
    MPO
    myeloperoxidase
    IBD
    inflammatory bowel disease
    MOPS
    3-(N-morpholino)-propanesulfonic acid
    pHi
    intracellular pH
    • Received August 19, 1997.
    • Accepted January 16, 1998.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics
Vol. 285, Issue 2
1 May 1998
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OtherGASTROINTESTINAL PHARMACOLOGY

Colitis-Induced Changes in the Expression of the Na+/H+ Exchanger Isoform NHE-1

Islam Khan, Farida M. Al-Awadi and Habib Abul
Journal of Pharmacology and Experimental Therapeutics May 1, 1998, 285 (2) 869-875;

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OtherGASTROINTESTINAL PHARMACOLOGY

Colitis-Induced Changes in the Expression of the Na+/H+ Exchanger Isoform NHE-1

Islam Khan, Farida M. Al-Awadi and Habib Abul
Journal of Pharmacology and Experimental Therapeutics May 1, 1998, 285 (2) 869-875;
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