Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherAUTONOMIC PHARMACOLOGY

Age-Related Decline in Beta Adrenergic and Adenosine A1 Receptor Function in the Heart Are Attenuated by Dietary Restriction

Erhe Gao, David L. Snyder, Jay Roberts, Eitan Friedman, Guoping Cai, Amir Pelleg and Joel Horwitz
Journal of Pharmacology and Experimental Therapeutics April 1998, 285 (1) 186-192;
Erhe Gao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David L. Snyder
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jay Roberts
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eitan Friedman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guoping Cai
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amir Pelleg
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joel Horwitz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Previously published reports from this laboratory have shown that the antiadrenergic effect of adenosine A1 agonists declines with age in the rat heart [Gao et al. (1997)J Mol Cell Cardiol 29:593–602] and that this decline may be caused by a decrease in coupling between adenosine A1 receptors (AdoA1R) and guanine nucleotide-binding proteins [Cai et al. (1997)Circ Res 81:1065–1071]. Dietary restriction (DR; 60% calories of ad libitum) has been shown to attenuate age-related changes in cellular signal transduction pathways. Therefore, the present study investigated whether DR altered the age-related changes in AdoA1R-mediated function in senescent rat hearts. Ventricular membranes were isolated from the hearts of ad libitum (AL) fed and DR male F344 rats that were 6, 12 and 24 months of age. In AL rats, there was an age-related decline in isoproterenol (ISO)-stimulated adenylyl cyclase when compared with the 6-month-old rats. The decline in ISO-stimulated cyclase was attenuated in DR animals. In AL rats, inhibition of ISO-stimulated adenylyl cyclase by the AdoA1R agonist, N6-p-sulfophenyladenosine (SPA) decreased with age. In DR rats, the age-related decline in inhibition was attenuated. Previous results from this laboratory indicated that in AL fed rats, there was an age-related decrease in the percentage of high-affinity binding sites for SPA, from 55% at 6 months to 23% at 24 months. Diet restriction attenuated this age-related shift in high-affinity binding sites so that the percentage of high-affinity sites at 24 months was 42%. Our results suggest that DR maintains AdoA1R function by preventing a loss of high-affinity AdoA1R sites.

Footnotes

  • Send reprint requests to: Dr. Joel Horwitz, Department of Pharmacology, Allegheny University of the Health Sciences, MCP ♦ Hahnemann School of Medicine, 3200 Henry Ave., Philadelphia, PA 19129.

  • ↵1 This study was supported in part by grants from the National Institutes of Health (KO7 AG 00532, AG 11060) and the Allegheny Health Education Research Foundation.

  • Abbreviations:
    DR
    diet restriction
    AL
    ad libitum
    AdoA1R
    adenosine A1 receptor
    ISO
    isoproterenol
    SPA
    N6-p-sulfophenyladenosine
    8-SPT
    8-p-sulfophenyltheophylline
    3H-DPCPX
    cyclopentyl-1,3-dipropylxanthine, 8-[dipropyl-2,3-3H], Gpp(NH)p-5′-guanylylimidodiphosphate
    EGTA
    ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid
    EDTA
    ethylenediaminetetraacetic acid
    HEPES
    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
    ANOVA
    analysis of variance
    CHAPS
    3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
    • Received June 18, 1997.
    • Accepted December 10, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 285, Issue 1
1 Apr 1998
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Age-Related Decline in Beta Adrenergic and Adenosine A1 Receptor Function in the Heart Are Attenuated by Dietary Restriction
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
OtherAUTONOMIC PHARMACOLOGY

Age-Related Decline in Beta Adrenergic and Adenosine A1 Receptor Function in the Heart Are Attenuated by Dietary Restriction

Erhe Gao, David L. Snyder, Jay Roberts, Eitan Friedman, Guoping Cai, Amir Pelleg and Joel Horwitz
Journal of Pharmacology and Experimental Therapeutics April 1, 1998, 285 (1) 186-192;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
OtherAUTONOMIC PHARMACOLOGY

Age-Related Decline in Beta Adrenergic and Adenosine A1 Receptor Function in the Heart Are Attenuated by Dietary Restriction

Erhe Gao, David L. Snyder, Jay Roberts, Eitan Friedman, Guoping Cai, Amir Pelleg and Joel Horwitz
Journal of Pharmacology and Experimental Therapeutics April 1, 1998, 285 (1) 186-192;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The Contribution of Classical (β1/2-) and Atypical β-Adrenoceptors to the Stimulation of Human White Adipocyte Lipolysis and Right Atrial Appendage Contraction by Novel β3-Adrenoceptor Agonists of Differing Selectivities
  • Mechanism of Gallbladder Relaxation in the Cat: Role of Norepinephrine ,
  • Gender Differences in the Expression of Endothelin Receptors in Human Saphenous Veins In Vitro
Show more AUTONOMIC PHARMACOLOGY

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics