Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherCARDIOVASCULAR PHARMACOLOGY

Lipid Transfer Protein I Facilitated Transfer of Cyclosporine from Low- to High-Density Lipoproteins is Only Partially Dependent on its Cholesteryl Ester Transfer Activity

Kishor M. Wasan, Manisha Ramaswamy, Wesley Wong and P. Haydn Pritchard
Journal of Pharmacology and Experimental Therapeutics February 1998, 284 (2) 599-605;
Kishor M. Wasan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Manisha Ramaswamy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wesley Wong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
P. Haydn Pritchard
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The purpose of this study was to determine if lipid transfer protein (LTP I) regulates the plasma lipoprotein distribution of cyclosporine (CSA). Experimental strategies that involved the supplementation and inhibition of LTP I were used to test these hypotheses. Incubation of CSA with human plasma supplemented with exogenous LTP I resulted in a significantly greater percentage of CSA recovered in the high-density lipoprotein (HDL)/lipoprotein deficient plasma (LPDP) fraction than in the low-density lipoprotein (LDL)/very low-density lipoprotein (VLDL) fraction compared to plasma which had no exogenous LTP I added. Incubation of radiolabeled cholesteryl ester (CE) or CSA-enriched HDL or LDL in T150 buffer supplemented with LTP I resulted in a significantly greater percentage of CE than CSA being transferred from HDL to LDL and LDL to HDL. However, the percent transfer from LDL to HDL was significantly lower for CE than CSA when these particles were incubated in LPDP that contained endogenous LTP I. The percent transfer of CE from HDL to LDL and LDL to HDL was significantly decreased in the presence of TP2, a monoclonal antibody directed against LTP I, compared to controls. The percent transfer of CSA from LDL to HDL was significantly decreased in the presence of TP2. However, the percent transfer of CSA from HDL to LDL in the presence of TP2 was not significantly different compared to controls. These findings suggest that the transfer of CSA between HDL and LDL is only partially facilitated through LTP I CE transfer activity.

Footnotes

  • Send reprint requests to: Dr. Kishor M. Wasan, Assistant Professor of Pharmaceutics, Faculty of Pharmaceutical Sciences, The University of British Columbia 2146 East Mall Vancouver, British Columbia, Canada V6T 1Z3.

  • ↵1 This work was supported with grants from the University of British Columbia Development Fund and the Medical Research Council of Canada (Grant MA-14484).

  • Abbreviations:
    LTP I
    lipid transfer protein I
    CE
    cholesteryl ester
    TG
    triglyceride
    AmpB
    amphotericin B
    HDL
    high-density lipoproteins
    LDL
    low density lipoproteins
    VLDL
    very low density lipoproteins
    CSA
    cyclosporine
    T 150 buffer
    50 mM Tris-HCl, 150 mM NaCl, 0.02% sodium azide, 0.01% disodium EDTA, pH 7.4
    TP2
    monoclonal antibody directed against lipid transfer protein I
    LPDP
    lipoprotein-deficient plasma
    EDTA
    ethylenediaminetetraacetic acid
    ELISA
    enzyme-linked immunosorbent assay
    PBS
    phosphate-buffered saline
    CMC
    carboxy-methylcellulose
    PC
    egg phosphatidylcholine
    k
    constant
    fraction of label transferred
    t, time
    • Received May 16, 1997.
    • Accepted October 28, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 284, Issue 2
1 Feb 1998
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Lipid Transfer Protein I Facilitated Transfer of Cyclosporine from Low- to High-Density Lipoproteins is Only Partially Dependent on its Cholesteryl Ester Transfer Activity
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
OtherCARDIOVASCULAR PHARMACOLOGY

Lipid Transfer Protein I Facilitated Transfer of Cyclosporine from Low- to High-Density Lipoproteins is Only Partially Dependent on its Cholesteryl Ester Transfer Activity

Kishor M. Wasan, Manisha Ramaswamy, Wesley Wong and P. Haydn Pritchard
Journal of Pharmacology and Experimental Therapeutics February 1, 1998, 284 (2) 599-605;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
OtherCARDIOVASCULAR PHARMACOLOGY

Lipid Transfer Protein I Facilitated Transfer of Cyclosporine from Low- to High-Density Lipoproteins is Only Partially Dependent on its Cholesteryl Ester Transfer Activity

Kishor M. Wasan, Manisha Ramaswamy, Wesley Wong and P. Haydn Pritchard
Journal of Pharmacology and Experimental Therapeutics February 1, 1998, 284 (2) 599-605;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Differential Contribution of Angiotensinergic and Cholinergic Receptors in the Hypothalamic Paraventricular Nucleus to Osmotically Induced AVP Release
  • Membrane Potential-Dependent and -Independent Vasodilation in Small Pulmonary Arteries from Chronically Hypoxic Rats
  • Effects of Gonadal Steroids on Ventricular Repolarization and on the Response to E4031
Show more CARDIOVASCULAR PHARMACOLOGY

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics