Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Signal Transduction Mechanisms Involved in Angiotensin-(1–7)-Stimulated Arachidonic Acid Release and Prostanoid Synthesis in Rabbit Aortic Smooth Muscle Cells

Mubarack M. Muthalif, Ibrahim F. Benter, Mohammad R. Uddin, Jason L. Harper and Kafait U. Malik
Journal of Pharmacology and Experimental Therapeutics January 1998, 284 (1) 388-398;
Mubarack M. Muthalif
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ibrahim F. Benter
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mohammad R. Uddin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jason L. Harper
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kafait U. Malik
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

This study investigated the signal transduction mechanisms of angiotensin-(1–7) [Ang-(1–7)]- and Ang II-stimulated arachidonic acid (AA) release for prostaglandin (PG) production in rabbit aortic vascular smooth muscle cells. Ang II and Ang-(1–7) enhanced AA release in cells prelabeled with [3H]AA. However, 6-keto-PGF1α synthesis produced by Ang II was much less than that caused by Ang-(1–7). In the presence of the lipoxygenase inhibitor baicalein, Ang II enhanced production of 6-keto-PGF1α to a greater degree than Ang-(1–7). Angiotensin type (AT)1 receptor antagonist DUP-753 inhibited only Ang II-induced [3H]AA release, whereas the AT2 receptor antagonist PD-123319 inhibited both Ang II- and Ang-(1–7)-induced [3H]AA release. Ang-(1–7) receptor antagonist d-Ala7-Ang-(1–7) inhibited the effect of Ang-(1–7), but not of Ang II. In cells transiently transfected with cytosolic phospholipase A2(cPLA2), mitogen-activated protein (MAP) kinase or Ca++-/calmodulin-dependent protein (CAM) kinase II antisense oligonucleotides, Ang-(1–7)- and Ang II-induced [3H]AA release was attenuated. The CaM kinase II inhibitor KN-93 and the MAP kinase kinase inhibitor PD-98059 attenuated both Ang-(1–7)- and Ang II-induced cPLA2 activity and [3H]AA release. Ang-(1–7) and Ang II also increased CaM kinase II and MAP kinase activities. Although KN-93 attenuated MAP kinase activity, PD-98059 did not affect CaM kinase II activity. Both Ang II and Ang-(1–7) caused translocation of cytosolic PLA2 to the nuclear envelope. These data show that Ang-(1–7) and Ang II stimulate AA release and prostacyclin synthesisvia activation of distinct types of AT receptors. Both peptides appear to stimulate CaM kinase II, which in turn,via MAP kinase activation, enhances cPLA2activity and release of AA for PG synthesis.

Footnotes

  • Send reprint requests to: Kafait U. Malik, Ph.D., D.Sc., Professor, Department of Pharmacology, College of Medicine, The University of Tennessee, Memphis, Memphis, TN 38163.

  • ↵1 This work was supported by USPHS-NIH grant 19134 from the National Heart, Lung, and Blood Institute (K.U.M.), an American Heart Association Tennessee Affiliate New Investigator Award (I.F.B.), and a Center for Neuroscience Fellowship and an American Heart Association Tennessee Affiliate Postdoctoral Fellowship (M.M.M.).

  • Abbreviations:
    AA
    arachidonic acid
    Ang
    angiotensin
    AT
    angiotensin type
    BSS
    balanced salt solution
    BSA
    bovine serum albumin
    CaM
    calmodulin
    CaM kinase
    Ca++-/CaM-dependent protein kinase
    cPLA2
    cytosolic PLA2
    DTT
    dithiothreitol
    EGTA
    ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid
    FBS
    fetal bovine serum
    FOX
    ferric oxidation of xylenol orange
    HBSS
    Hanks’ balanced salt solution
    HEPES
    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
    LO
    lipoxygenase
    MAFP
    methyl arachidonyl fluorophosphonate
    MAP kinase
    mitogen-activated protein kinase
    MEK
    MAP kinase kinase
    PG
    prostaglandin
    PK
    protein kinase
    PL
    phospholipase
    PMSF
    phenylmethylsulfonyl fluoride
    TBS
    tris-buffered saline
    TBST
    tris-buffered saline with Tween-20
    TRITC
    tetramethyl rhodamine B isothiocyanate
    VSMC
    vascular smooth muscle cells
    • Received June 30, 1997.
    • Accepted September 29, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 284, Issue 1
1 Jan 1998
  • Table of Contents
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Signal Transduction Mechanisms Involved in Angiotensin-(1–7)-Stimulated Arachidonic Acid Release and Prostanoid Synthesis in Rabbit Aortic Smooth Muscle Cells
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Signal Transduction Mechanisms Involved in Angiotensin-(1–7)-Stimulated Arachidonic Acid Release and Prostanoid Synthesis in Rabbit Aortic Smooth Muscle Cells

Mubarack M. Muthalif, Ibrahim F. Benter, Mohammad R. Uddin, Jason L. Harper and Kafait U. Malik
Journal of Pharmacology and Experimental Therapeutics January 1, 1998, 284 (1) 388-398;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Signal Transduction Mechanisms Involved in Angiotensin-(1–7)-Stimulated Arachidonic Acid Release and Prostanoid Synthesis in Rabbit Aortic Smooth Muscle Cells

Mubarack M. Muthalif, Ibrahim F. Benter, Mohammad R. Uddin, Jason L. Harper and Kafait U. Malik
Journal of Pharmacology and Experimental Therapeutics January 1, 1998, 284 (1) 388-398;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Antagonism of an Adenosine/ATP Receptor in FollicularXenopus Oocytes
  • Toxin and Subunit Specificity of Blocking Affinity of Three Peptide Toxins for Heteromultimeric, Voltage-Gated Potassium Channels Expressed in Xenopus Oocytes
  • Inhibition of Voltage-Dependent Sodium Channels by the Anticonvulsant γ-Aminobutyric Acid Type A Receptor Modulator, 3-Benzyl-3-Ethyl-2-Piperidinone
Show more CELLULAR AND MOLECULAR PHARMACOLOGY

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics