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OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Ethanol Increases the Activity of Ca++-Dependent K+ (mslo) Channels: Functional Interaction with Cytosolic Ca++ 

Alejandro M. Dopico, Vellareddy Anantharam and Steven N. Treistman
Journal of Pharmacology and Experimental Therapeutics January 1998, 284 (1) 258-268;
Alejandro M. Dopico
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Vellareddy Anantharam
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Steven N. Treistman
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Abstract

Ethanol (EtOH) reversibly activates large conductance, Ca++-activated K+ (BK) channels in rat neurohypophysial terminals, an effect that probably contributes to the inhibition of vasopressin release by this drug. Heterogeneity in the terminal channel population makes it difficult to determine the mechanisms underlying this activation. Here, we report the effects of EtOH on the steady-state activity of BK channels cloned from mouse brain (mslo, α subunit) and expressed inXenopus oocytes. EtOH reversibly increased mslochannel activity in excised patches, showing a potency (EC50 = 24 mM) similar to that reported using native channels. EtOH activation was observed under conditions that make it highly improbable that it is mediated by freely diffusible second messengers, or secondary to G-protein modulation. Rather, it probably results from a functional interaction between the drug and the channel α subunit. Activation occurred without increase in the number of functional channels present in the patch and resulted from actions that were a function of EtOH concentration: at ≤10 mM, activation was due to a decrease in the channel mean closed time, whereas between 25 and 100 mM, activation was due to both a decrease in the mean closed time and an increase in the mean open time. The characteristic high unitary conductance and ionic selectivity of BK channels were unaltered by the drug. Whereas the voltage dependence of channel gating remained unchanged, channel activation mediated by the response of the Ca++-sensing site(s) to increases in the concentration of intracellular calcium, [Ca++]ic, was reduced by EtOH. This finding is consistent with EtOH and [Ca++]ic behaving functionally as partial and full agonists of mslo channels, respectively. Because the potentiation of mslo activity by the drug decreased as Ca++ levels were increased, EtOH-activation of BK channels would be most evident when [Ca++]ic is near resting levels, rather than during periods of high activity and Ca++ influx.

Footnotes

  • Send reprint requests to: Steven N. Treistman, Ph.D., Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655.

  • ↵1 This work was supported by grants from the Alcoholic Beverage Medical Research Foundation (A.M.D.) and National Institutes of Health AA08003 (S.N.T.).

  • ↵2 Present affiliation: Department of Physiological Sciences, University of California at Los Angeles, CA 90095.

  • Abbreviations:
    EtOH
    ethanol
    BK
    large conductance, Ca++-activated K+
    CAK
    Ca++-dependent K+
    [Ca++]ic
    intracellular calcium
    EGTA
    10 ethylene glycol-bis(β-aminoethyl ether) N,N,N′,N′-tetraacetic acid
    I/O
    inside-out
    N
    number of functional channels present in the patch membrane
    Po
    probability that a particular channel is open
    T
    period of recording
    to
    channel mean open time
    #o
    number of channel openings during T
    tc
    channel mean closed time
    Pc
    probability that a particular channel is closed
    #c
    number of channel closures during T
    γ
    single-channel conductance
    i
    unitary current amplitude
    • Received April 10, 1997.
    • Accepted September 15, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics
Vol. 284, Issue 1
1 Jan 1998
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OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Ethanol Increases the Activity of Ca++-Dependent K+ (mslo) Channels: Functional Interaction with Cytosolic Ca++ 

Alejandro M. Dopico, Vellareddy Anantharam and Steven N. Treistman
Journal of Pharmacology and Experimental Therapeutics January 1, 1998, 284 (1) 258-268;

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OtherCELLULAR AND MOLECULAR PHARMACOLOGY

Ethanol Increases the Activity of Ca++-Dependent K+ (mslo) Channels: Functional Interaction with Cytosolic Ca++ 

Alejandro M. Dopico, Vellareddy Anantharam and Steven N. Treistman
Journal of Pharmacology and Experimental Therapeutics January 1, 1998, 284 (1) 258-268;
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