Abstract
The role of Ca++ in cell death is controversial. Extracellular Ca++ influx and calpain activation occurred during the late phase of renal proximal tubule cell injury produced by the mitochondrial inhibitor antimycin A. Chelation of intracellular Ca++, extracellular Ca++, the calcium channel blocker nifedipine, calpain inhibitor 1 and the dissimilar calpain inhibitor PD150606 blocked antimycin A-induced influx of extracellular Ca++ and cell death. The calcium channel blocker verapamil was ineffective. Calpain inhibitor 1 and PD150606 were cytoprotective also against tetrafluoroethyl-l-cysteine-, bromohydroquinone-, oxidant (t-butylhydroperoxide)- and calcium ionophore (ionomycin)-induced cell death. Extracellular Ca++ influx was associated with the translocation of calpain activity from the cytosol to the membrane and was prevented by calpain inhibitor 1, PD150606 and nifedipine. Finally, nifedipine, calpain inhibitor 1, PD150606 and the Cl− channel inhibitors [5-nitro-2-(3-phenylpropylamino)-benzoate, niflumic acid, diphenylamine-2-carboxylate, and indanyloxyacetic acid] blocked the increase in Cl− influx that occurs during the late phase of cell injury and triggers terminal cell swelling and death. These data suggest that Ca++ and calpains play a common and critical role in renal proximal tubule cell death produced by diverse agents. In addition, calpain activation appears to play a dual role during the late phase of cell injury. Initial calpain activation elicits extracellular Ca++ influx through a nifedipine-sensitive pathway, resulting in calpain translocation to the membrane and in turn Cl− influx.
Footnotes
-
Send reprint requests to: Rick G. Schnellmann, Ph.D., Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, 4301 W. Markham St., Slot 638, Little Rock, AR 72205-7199. E-mail: rschnell{at}biomed.uams.edu.
-
↵1 S. L. W. was supported by an American Heart Association, Arkansas Affiliate, Predoctoral Fellowship.
-
↵2 Portions of this work were presented at the XIIth International Congress of Pharmacology, Montreal, Canada, on July 24–26, 1994; 6th Congress on Nephrotoxicity and Nephrocarcinogenicity in Noordwijkhout, Netherlands, on September 22–24, 1994; 28th Annual Meeting of the American Society of Nephrology, San Diego, CA, on November 5–8, 1995; and 36th Annual Meeting of the Society of Toxicology, Cincinnati, OH, on March 9–13, 1997.
-
↵3 S. L. Waters and S. S. Sarang contributed equally.
-
↵4 Present address: Department of Neuroscience Therapeutics, Parke-Davis Pharmaceutical Research Division, Division of Warner-Lambert Company, 2800 Plymouth Rd., Ann Arbor, MI 48105.
- Abbreviations:
- RPT
- renal proximal tubules
- LDH
- lactate dehydrogenase
- PD150606
- 3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid
- SLLVY-AMC
- N-succinyl-Leu-Leu-Val-Tyr-AMC
- NPPB
- 5-nitro-2-(3-phenylpropylamino)-benzoate
- DPC
- diphenylamine-2-carboxylate
- IAA-94
- indanyloxyacetic acid
- Received April 29, 1997.
- Accepted August 22, 1997.
- The American Society for Pharmacology and Experimental Therapeutics
JPET articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|