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OtherENDOCRINE PHARMACOLOGY

Inhibition of Bufalin on Pituitary and Testicular Function in Rats

Shyi-Wu Wang, Yu-Chung Chiao, Shiow-Chwen Tsai, Chien-Chen Lu, Jiann-Jong Chen, Hsi-Chang Shih, Yen-Hao Chen, Ho Lin, Chii-Min Hwu and Paulus S. Wang
Journal of Pharmacology and Experimental Therapeutics November 1997, 283 (2) 528-532;
Shyi-Wu Wang
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Yu-Chung Chiao
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Shiow-Chwen Tsai
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Chien-Chen Lu
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Jiann-Jong Chen
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Hsi-Chang Shih
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Yen-Hao Chen
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Ho Lin
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Chii-Min Hwu
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Paulus S. Wang
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Abstract

The effects of bufalin on the secretion of testosterone and luteinizing hormone (LH) and the accumulation of testicular adenosine 3′:5′-cyclic monophosphate (cAMP) were studied. Male rats were injected with bufalin, human chorionic gonadotropin (hCG), gonadotropin releasing hormone (GnRH), hCG plus bufalin or GnRH plus bufalinvia a jugular catheter. Blood samples were collected at several intervals subsequent to the challenge. In the in vitro study, rat testis blocks were incubated with bufalin, hCG or both for 1 h. The anterior pituitary gland was incubated with bufalin, GnRH or both for 30 min. The media were analyzed for testosterone or LH. For studying cAMP accumulation, testicular blocks were incubated for 1 h with the medium containing isobutyl-1-methylxanthine. After incubation, tissues were extracted by ethanol before measuring cAMP concentration. A single intravenous injection of bufalin decreased the basal and hCG-stimulated levels of plasma testosterone. Administration of bufalin in vitroresulted in an inhibition of both basal and hCG-stimulated release of testosterone. Bufalin diminished cAMP accumulation in rat testes. However, the basal levels of plasma and medium LH were not altered by bufalin administration. Likewise, the LH response to GnRH was diminished by bufalin administration, both in vivo andin vitro. These results suggest that the inhibition of testosterone production by bufalin is partly caused by a decrease of testicular cAMP accumulation and LH response to GnRH in rats.

Footnotes

  • Send reprint requests to: Shyi-Wu Wang, PhD, Department of Physiology, Chang Gung College of Medicine and Technology, Tao-Yuan, Taiwan, Republic of China.

  • ↵1 This study was supported by the grants from Chang Gung College of Medicine and Technology (CMRP 504), the Department of Health, the Executive Yuan (DOH85-CM-032) and National Science Council (NSC84–2331-B-182–101), Taiwan, R.O.C.

  • Abbreviations:
    cAMP
    adenosine 3′:5′-cyclic monophosphate
    hCG
    human chorionic gonadotropin
    LH
    luteinizing hormone
    RIA
    radioimmunoassay
    IBMX
    isobutyl-1-methylxanthine
    GnRH
    gonadotropin releasing hormone
    HEPES
    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
    • Received January 10, 1997.
    • Accepted July 15, 1997.
  • The American Society for Pharmacology and Experimental Therapeutics
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Journal of Pharmacology and Experimental Therapeutics
Vol. 283, Issue 2
1 Nov 1997
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OtherENDOCRINE PHARMACOLOGY

Inhibition of Bufalin on Pituitary and Testicular Function in Rats

Shyi-Wu Wang, Yu-Chung Chiao, Shiow-Chwen Tsai, Chien-Chen Lu, Jiann-Jong Chen, Hsi-Chang Shih, Yen-Hao Chen, Ho Lin, Chii-Min Hwu and Paulus S. Wang
Journal of Pharmacology and Experimental Therapeutics November 1, 1997, 283 (2) 528-532;

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OtherENDOCRINE PHARMACOLOGY

Inhibition of Bufalin on Pituitary and Testicular Function in Rats

Shyi-Wu Wang, Yu-Chung Chiao, Shiow-Chwen Tsai, Chien-Chen Lu, Jiann-Jong Chen, Hsi-Chang Shih, Yen-Hao Chen, Ho Lin, Chii-Min Hwu and Paulus S. Wang
Journal of Pharmacology and Experimental Therapeutics November 1, 1997, 283 (2) 528-532;
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