Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Abstract

Leukotriene C4 receptors on guinea pig tracheocytes.

S Prié, G Guillemette, G Boulay, P Borgeat and P Sirois
Journal of Pharmacology and Experimental Therapeutics October 1995, 275 (1) 312-318;
S Prié
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G Guillemette
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G Boulay
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
P Borgeat
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
P Sirois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Leukotriene (LT) C4 receptors have been characterized on freshly isolated guinea pig tracheal epithelial cells (tracheocytes). The [3H]LTC4 receptor affinity was enhanced by increasing the sodium (60-160 mM) and the magnesium (0-10 mM) concentrations. Low concentrations of calcium (0-3 mM) increased [3H]LTC4 binding, but high concentrations (3-10 mM) decreased it. The pH (6.5-8.0) had no effect on [3H]LTC4 binding to tracheocytes. Under our experimental conditions, binding equilibrium was reached after 20 min. The association and the dissociation rate constants were estimated to be 2.75 +/- 0.25 x 10(6) M-1.min-1 and 0.093 +/- 0.008 min-1, respectively. The Kd (35.4 +/- 8.6 nM) and the Bmax values (2.4 +/- 0.6 x 10(5) receptors/cell) were determined by Scatchard analysis. LTB4, LTD4 and LTE4 did not inhibit [3H]LTC4 binding to the receptors. However, the compound FPL 55712 inhibited the binding of [3H]LTC4 with an IC50 value of 9.0 +/- 1.0 microM. [3H]LTC4 was not metabolized during the binding assays, as confirmed by reverse-phase high-performance liquid chromatography. The lack of [3H]LTC4 binding to glutathione-S-transferase was demonstrated in the presence of an excess of reduced glutathione. LTC4 produced a concentration-dependent increase of free Ca++ in tracheocytes. Our results suggest that guinea pig tracheocytes possess a specific LTC4 receptor coupled to a Ca++ signaling pathway. This LTC4 receptor may play a key role in the epithelium-dependent responses of airway smooth muscle.

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 275, Issue 1
1 Oct 1995
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Leukotriene C4 receptors on guinea pig tracheocytes.
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Leukotriene C4 receptors on guinea pig tracheocytes.

S Prié, G Guillemette, G Boulay, P Borgeat and P Sirois
Journal of Pharmacology and Experimental Therapeutics October 1, 1995, 275 (1) 312-318;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Leukotriene C4 receptors on guinea pig tracheocytes.

S Prié, G Guillemette, G Boulay, P Borgeat and P Sirois
Journal of Pharmacology and Experimental Therapeutics October 1, 1995, 275 (1) 312-318;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics