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Abstract

IL-1 beta modulates the concanavalin-A-induced expression of proenkephalin A mRNA in murine thymocytes.

K M Linner, S E Nicol and B M Sharp
Journal of Pharmacology and Experimental Therapeutics December 1993, 267 (3) 1566-1572;
K M Linner
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S E Nicol
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B M Sharp
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Abstract

We have previously shown that proenkephalin A (PEA) messenger RNA (mRNA) is induced in murine thymocytes by the T cell-specific mitogen concanavalin-A (Con-A). We now show that this Con-A-induced expression of PEA mRNA is modulated by the cytokine murine interleukin-1 beta (mIL-1 beta) in a biphasic, dose-dependent manner. Murine thymocytes were cultured for 72 h with Con-A and with varying concentrations of mIL-1 beta. PEA mRNA expression was analyzed by Northern gel and solution hybridization techniques. Concentrations of mIL-1 beta of 10(-14) and 10(-13) M enhanced the Con-A-induced expression of PEA mRNA in cultured murine thymocytes up to 2.5-fold, whereas higher concentrations of mIL-1 beta (10(-11) and 10(-10) M) inhibited its expression 60 and 85%, respectively. Both the enhancing and inhibiting effects of mIL-1 beta in the Con-A-induced expression of PEA mRNA were reversed by a 100-fold excess of interleukin-1 receptor antagonist protein, but not by a 10-fold excess of interleukin-1 receptor antagonist protein. The effects of mIL-1 beta on PEA mRNA expression in Con-A-activated thymocytes are different from its effects on Con-A-stimulated thymocyte proliferation. In the latter case, only enhancement of thymocyte proliferation was seen, as measured by [3H]thymidine incorporation. The present study demonstrates that PEA mRNA expression is regulated by IL-1 beta, which is thought to play a role in thymocyte maturation.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 267, Issue 3
1 Dec 1993
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Abstract

IL-1 beta modulates the concanavalin-A-induced expression of proenkephalin A mRNA in murine thymocytes.

K M Linner, S E Nicol and B M Sharp
Journal of Pharmacology and Experimental Therapeutics December 1, 1993, 267 (3) 1566-1572;

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Abstract

IL-1 beta modulates the concanavalin-A-induced expression of proenkephalin A mRNA in murine thymocytes.

K M Linner, S E Nicol and B M Sharp
Journal of Pharmacology and Experimental Therapeutics December 1, 1993, 267 (3) 1566-1572;
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