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Abstract

Transport of salicylic acid through monolayers of a kidney epithelial cell line (LLC-PK1).

J Y Chatton and F Roch-Ramel
Journal of Pharmacology and Experimental Therapeutics May 1992, 261 (2) 518-524;
J Y Chatton
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F Roch-Ramel
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Abstract

LLC-PK1 cells were used as a model of renal proximal epithelium to study the nonionic diffusion of salicylic acid (SAL). The apparent [14C]SAL transcellular permeability (PSal) and intracellular content were estimated at 20-21 degrees C from fluxes measured across cell monolayers grown on filters, in both apical-to-basolateral and basolateral-to-apical directions. The medium pH of the cis-side was varied from 6.0 to 7.4, and the medium pH of the trans-side was kept at 7.4. In the apical-to-basolateral direction, PSal increased linearly with the calculated concentration of nonionized SAL, indicating that SAL permeability was essentially the result of nonionic diffusion. In the basolateral-to-apical direction, PSal was about 2.5-fold higher than in the apical-to-basolateral direction and was not linearly related to the concentration of nonionized SAL molecules (0-4.5 nM), suggesting that besides nonionic diffusion, SAL was transported in its ionized form by a facilitated mechanism still active at 21 degrees C. This was confirmed by measuring basolateral-to-apical fluxes at 37 degrees C and observing that probenecid, an inhibitor of organic anion secretion, and cold SAL decreased PSal. Interestingly, at 37 degrees C, PSal in the apical-to-basolateral direction was also decreased by probenecid and cold SAL, suggesting the existence of a facilitated transport in this direction. These data demonstrated that the secretory transport of SAL is present in LLC-PK1 cells. The facilitated transport observed in the apical-to-basolateral direction suggests that in proximal tubule, SAL reabsorption might occur by facilitated mechanism and nonionic diffusion.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 261, Issue 2
1 May 1992
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Abstract

Transport of salicylic acid through monolayers of a kidney epithelial cell line (LLC-PK1).

J Y Chatton and F Roch-Ramel
Journal of Pharmacology and Experimental Therapeutics May 1, 1992, 261 (2) 518-524;

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Abstract

Transport of salicylic acid through monolayers of a kidney epithelial cell line (LLC-PK1).

J Y Chatton and F Roch-Ramel
Journal of Pharmacology and Experimental Therapeutics May 1, 1992, 261 (2) 518-524;
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