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Abstract

Alpha-1 adrenergic receptor: binding and phosphoinositide breakdown in human myometrium.

M Breuiller-Fouche, F Doualla-Bell Kotto Maka, B Geny and F Ferre
Journal of Pharmacology and Experimental Therapeutics July 1991, 258 (1) 82-87;
M Breuiller-Fouche
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F Doualla-Bell Kotto Maka
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B Geny
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F Ferre
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Abstract

Alpha-1 adrenergic receptors were examined in both inner and outer layers of human pregnant myometrium using radioligand binding of [3H]prazosin. [3H]prazosin bound rapidly and reversibly to a single class of high affinity binding sites in myometrial membrane preparations. Scatchard analysis gave similar values of equilibrium dissociation constants in both myometrial layers. In contrast, more alpha-1 adrenergic receptors were detected in the outer layer than in the inner layer. Antagonist inhibited [3H]prazosin binding with an order of potency of prazosin greater than phentolamine greater than idazoxan. Competition experiments have also revealed that a stable guanine nucleotide decreases the apparent affinity of norepinephrine for myometrial [3H]prazosin binding sites. The functional status of these alpha-1 adrenergic receptors was also assessed by measuring the norepinephrine-induced accumulation of inositol phosphates in myometrial tissue. Norepinephrine produced a concentration-dependent accumulation of inositol phosphates in both myometrial layers. However, norepinephrine-induced increases in inositol 1,4,5-triphosphate were only observed in the outer layer. These results indicate that alpha-1 adrenergic receptors in human myometrium at the end of pregnancy are linked to phosphoinositide hydrolysis and that this response occurs mainly in the outer layer.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 258, Issue 1
1 Jul 1991
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Abstract

Alpha-1 adrenergic receptor: binding and phosphoinositide breakdown in human myometrium.

M Breuiller-Fouche, F Doualla-Bell Kotto Maka, B Geny and F Ferre
Journal of Pharmacology and Experimental Therapeutics July 1, 1991, 258 (1) 82-87;

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Abstract

Alpha-1 adrenergic receptor: binding and phosphoinositide breakdown in human myometrium.

M Breuiller-Fouche, F Doualla-Bell Kotto Maka, B Geny and F Ferre
Journal of Pharmacology and Experimental Therapeutics July 1, 1991, 258 (1) 82-87;
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