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Journal of Pharmacology and Experimental Therapeutics

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Abstract

Cellular mechanisms of somatostatin inhibition of calcium influx in the anterior pituitary cell line AtT-20.

T Reisine
Journal of Pharmacology and Experimental Therapeutics August 1990, 254 (2) 646-651;
T Reisine
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Abstract

The cellular mechanisms by which the hypothalamic peptide somatostatin (SRIF) inhibits Ca+(+) influx were investigated in the pituitary cell line AtT-20. Cytosolic Ca+(+) levels were measured using the fluorescent probe Quin 2. Calcium influx was stimulated by the Ca+(+) channel agonist Bay K 8644. Bay K 8644 increased Ca+(+) influx in a concentration-dependent manner and the stimulation of Ca+(+) influx was blocked by the Ca+(+) channel antagonists nifedipine and nitrendipine. SRIF analogs also blocked Bay K 8644-stimulated Ca+(+) influx. The rank order of potency of the analogs (SRIF-28 greater than D-Trp8-SRIF greater than SRIF) suggests that the effects of SRIF are mediated by SRIF-28 preferring receptors. Pretreatment of AtT-20 cells with pertussis toxin abolished SRIF's inhibition of Bay K 8644-evoked Ca+(+) influx suggesting that G proteins mediate the inhibitory effects of SRIF on Ca+(+) influx. The K+ channel antagonists tetraethylammonium, 4-aminopyridine and CsCl all stimulated Ca+(+) influx into AtT-20 cells. These agents did not alter Bay K 8644-evoked Ca+(+) influx or did they affect the ability of SRIF to inhibit Ca+(+) influx. Tetrodotoxin, the sodium channel blocker which inhibits action potential generation in AtT-20 cells, lowered basal Ca+(+) levels in AtT-20 cells but did not modify SRIF's inhibition of Bay K 8644-stimulated Ca+(+) influx. These findings suggest that SRIF receptors, linked directly to Ca+(+) channels via G proteins, may mediate SRIF's inhibition of Ca+(+) influx.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 254, Issue 2
1 Aug 1990
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Abstract

Cellular mechanisms of somatostatin inhibition of calcium influx in the anterior pituitary cell line AtT-20.

T Reisine
Journal of Pharmacology and Experimental Therapeutics August 1, 1990, 254 (2) 646-651;

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Abstract

Cellular mechanisms of somatostatin inhibition of calcium influx in the anterior pituitary cell line AtT-20.

T Reisine
Journal of Pharmacology and Experimental Therapeutics August 1, 1990, 254 (2) 646-651;
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