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Abstract

Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture.

M N Gillespie, J O Owasoyo, I F McMurtry and R F O'Brien
Journal of Pharmacology and Experimental Therapeutics February 1986, 236 (2) 339-343;
M N Gillespie
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J O Owasoyo
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I F McMurtry
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R F O'Brien
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Abstract

Recent reports have shown that cultured endothelial cells release into the culture medium a substance (or substances) that contracts isolated preparations of arterial smooth muscle [endothelial-derived constrictor factor (EDCF)]. To evaluate the coronary and cardiac effects of EDCF, isolated rabbit hearts retroperfused in a nonrecirculating system with Krebs-Henseleit solution were challenged with bolus injections (100-600 microliter) of either serum-free minimum essential medium (vehicle) or aortic endothelial cell supernates concentrated in minimum essential medium (EDCF). EDCF, but not its vehicle, produced dose-dependent coronary vasoconstriction unaccompanied by changes in left ventricular contraction or heart rate. The constrictor responses were remarkably well sustained with little or no decrement in resistance occurring over a 15-min observation period. Nitroprusside inhibited the development of and reversed on-going vasoconstriction evoked by EDCF. Neither meclofenamate nor diethylcarbamazine influenced EDCF-induced pressor responses, thereby negating a role for arachidonic acid metabolites. Coronary vasoconstriction induced by EDCF also was unaffected by blockade of alpha-adrenergic, histaminergic or serotonergic receptors. Incubation of EDCF with trypsin attenuated the pressor effects markedly, suggesting that EDCF may be a peptide. Roles for angiotensin or substance P were ruled out, however, as saralasin failed to influence EDCF-induced constriction and since substance P was inactive in the perfused rabbit heart preparation. We conclude that a substance (or substances), probably a peptide, released from cultured endothelial cells provokes sustained coronary vasoconstriction by an unknown mechanism.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 236, Issue 2
1 Feb 1986
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Abstract

Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture.

M N Gillespie, J O Owasoyo, I F McMurtry and R F O'Brien
Journal of Pharmacology and Experimental Therapeutics February 1, 1986, 236 (2) 339-343;

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Abstract

Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture.

M N Gillespie, J O Owasoyo, I F McMurtry and R F O'Brien
Journal of Pharmacology and Experimental Therapeutics February 1, 1986, 236 (2) 339-343;
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