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Journal of Pharmacology and Experimental Therapeutics

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Abstract

Studies on the structural prerequisites for the activation of the beta-endorphin receptor on the rat vas deferens.

J P Huidobro-Toro, E M Caturay, N Ling, N M Lee, H H Loh and E L Way
Journal of Pharmacology and Experimental Therapeutics July 1982, 222 (1) 262-269;
J P Huidobro-Toro
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E M Caturay
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N Ling
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N M Lee
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H H Loh
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E L Way
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Abstract

The inhibitory activity of a variety of opioid peptides was tested on the electrically stimulated rat vas deferens. Human beta-endorphin (beta h-EP) was the most potent of the opoioid-like peptides; it produced half-maximal inhibition of the neuromuscular twitching at a concentration of about 100 nM. The potency of beta h-EP was greater than that of camel, porcine, ovine or leucine5 beta h-EP, alpha-N-acetyl beta-ovine-EP was inactive. In contrast to beta-endorphin (beta-EP), methionine and leucine-enkephalin, dynorphin-(1-13), morphine and other narcotic analgesics were devoid of opioid-like activity. Fragments of beta-EP with amino acid deletions at the carboxy end of the molecule were considerably less potent than the parent compound. The fragment beta h-EP 1-21 was 70 times less potent than beta h-EP, whereas the segments beta h-EP 1-19 and beta h-EP 1-16 were both completely inactive. Deletions at the amino terminal of beta-EP yielded inactive compounds. The potency of beta h-EP was reduced in a dose-related fashion by applications of nanomolar concentrations of naloxone or N-allylnormetazocine and by micromolar concentrations of levorphanol or morphine. In decided contrast, opioid peptides such as methionine enkephalin, dynorphin-(1-13), beta-casomorphan derivatives or short chain beta h-EP segments such as beta-EP 1-16, beta-EP 1-19, beta-EP 6-31, beta-EP (1-5)-(16-31), methionine enkephalin plus beta-EP 6-31 or the N-acetyl beta-bovine-EP did not antagonize the inhibitory action of beta h-EP. The present results demonstrate that the rat vas deferens contains opioid receptors with considerable selectivity for beta-EP. It is concluded from this structure activity relationship study that the activation of the beta-EP receptor involves at least two sites of recognition on the beta-EP structure.

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Journal of Pharmacology and Experimental Therapeutics
Vol. 222, Issue 1
1 Jul 1982
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Abstract

Studies on the structural prerequisites for the activation of the beta-endorphin receptor on the rat vas deferens.

J P Huidobro-Toro, E M Caturay, N Ling, N M Lee, H H Loh and E L Way
Journal of Pharmacology and Experimental Therapeutics July 1, 1982, 222 (1) 262-269;

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Abstract

Studies on the structural prerequisites for the activation of the beta-endorphin receptor on the rat vas deferens.

J P Huidobro-Toro, E M Caturay, N Ling, N M Lee, H H Loh and E L Way
Journal of Pharmacology and Experimental Therapeutics July 1, 1982, 222 (1) 262-269;
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