Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Abstract

Tracer microinjection studies of prostaglandin E-2 transport in the rat nephron.

M L Kauker
Journal of Pharmacology and Experimental Therapeutics April 1975, 193 (1) 274-280;
M L Kauker
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Urinary excretion and tubular handling of 3-H-prostaglandin E2 (3-H-PGE2) were examined in anesthetized rats made diuretic with 2.5% saline infusion using the tracer microinjection technique. Radioactive inulin and PGE2 were simultaneously injected into surface convolutions of proximal and distal tubules with volumetric micropipettes and their urinary excretion were measured. The rate of microinjection was adjusted to approximate tubular flow. Net 3-H-PGE2 recoveries from the microinjected kidney were similar following injections into early (61.2 plus or minus 6.8%) and late (62.9 plus or minus 7.9%) proximal tubules, but significantly higher after injection into distal tubules (91.3 plus or minus 7.0%) indicating absorption of 3-H-PGE2 in the loop of Henle but not in the proximal and distal nephron. Excretion was primarily direct, that is, parallel with inulin excretion when PGE2 was deposited in distal convoluted tubules. After injection of 3-H-PGE2 into early and late proximal tubules, half of net recovery was direct and half showed temporal dissociation from inulin (indirect recovery), revealing retardation of PGE2 excretion in the loop. Segmental tubular efflux occurred primarily in the loop of Henle (48.5%). It was low in the distal nephron (13.5%) and negligible in the proximal convoluted tubule (6.1%). These studies indicate that the loop of Henle is the principal site of transtubular PGE2 movement. They do not suggest a primary tubular route of transfer of medullary prostaglandins to cortical vascular or peritubular sites of action except for possible inhibition of distal reabsorption from the luminal side.

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 193, Issue 1
1 Apr 1975
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Tracer microinjection studies of prostaglandin E-2 transport in the rat nephron.
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Tracer microinjection studies of prostaglandin E-2 transport in the rat nephron.

M L Kauker
Journal of Pharmacology and Experimental Therapeutics April 1, 1975, 193 (1) 274-280;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Tracer microinjection studies of prostaglandin E-2 transport in the rat nephron.

M L Kauker
Journal of Pharmacology and Experimental Therapeutics April 1, 1975, 193 (1) 274-280;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics