Abstract
The in vitro characteristics of the uptake of nonutilizable hexoses 2-deoxyglucose (2dG) and 3-O-methylglucose (3mG) and a nonutilizable amino acid, α-aminoisobutyric acid (AIB) by the isolated epithelial or muscle layers of the guinea-pig seminal vesicle were examined. The characteristics of 3mG uptake did not differ greatly in the two cell lines; it seemed to enter the cells of the muscle and epithelium by diffusion. The free form of 2dG was apparently actively transported into the epithelium, but appeared to enter the cells of the muscle by simple diffusion. 2dG was phosphorylated by both the epithelium and muscle. AIB was probably actively transported into the cells of both the epithelium and muscle, but to a greater extent in the epithelium. Neither the H2O contents nor the extracellular spaces of the muscle layer nor the epithelial layer of the guinea-pig seminal vesicle was different.
Footnotes
- Received December 13, 1972.
- Accepted June 14, 1973.
- © 1973 by The Williams & Wilkins Co.
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