Abstract
Recent reports have demonstrated that the antischistosomal drug hycanthone can have mutagenic, teratogenic and hepatotoxic properties. In this study, the effects on hepatic microsomes were investigated after a single i.m. injection of hycanthone or two of its chlorinated analogs to male rats. Two days after treatment with 100 mg/kg of hycanthone, aminopyrine demethylation was decreased by 48% and cytochrome P-450 by 34%. Aminopyrine demethylation remained approximately 50% of control values through the 7th day after hycanthone injection. Enzyme activity returned to control levels by the 14th day. Microsomal protein was decreased by hycanthone treatment and this was attributable to decreased smooth endoplasmic reticulum protein. Effects of hycanthone on other microsomal enzymes such as glucuronyltransferase, β-glucuronidase and acid phosphatase were not as great as those observed on the mixed function oxidase components. IA-4 (6-chloroindazole analog of hycanthone) did not markedly affect any of the microsomal parameters tested, whereas IA-3 (6-chloroindazole analog of lucanthone) decreased aminopyrine demethylation by 38% in smooth endoplasmic reticulum. Dose-response studies demonstrated that aminopyrine demethylation and cytochrome P-450 were significantly decreased by a single hycanthone injection at 50 or 100 mg/kg. A dose of 25 mg/kg caused only slight decreases in these parameters.
Footnotes
- Received February 13, 1973.
- Accepted May 10, 1973.
- © 1973 by The Williams & Wilkins Co.
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