Abstract
The disposition of carboxyl-14C-acetohydroxamic acid was evaluated in mice by comparison with 14C-urea, by using radioisotopic, spectrophotometric and chromatographic assays. Acetohydroxamate was absorbed about half as fast as urea, giving peak blood levels at one hour and had an approximate biologic half-life of four hours, about 3 times that of urea. Approximate drug space was 71% of body weight, suggesting that the compound was distributed throughout total body water. Within 24 hours, about 60% of the administered dose was excreted unchanged in the urine, 15 to 20% was excreted in the urine as acetamide and 10% was excreted as acetate, identified by dual isotope analysis. Another 7% was expired as carbon dioxide, presumably derived from acetate, while about 2% remained in the blood and tissues with rather uniform distribution and only 1% appeared in the feces. The kinetic pattern of urinary excretion of acetate and acetamide suggested that the acetate was derived directly from the acetohydroxamate and not secondarily from the acetamide.
Footnotes
- Received August 10, 1972.
- Accepted March 21, 1973.
- © 1972 by The Williams & Wilkins Co.
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