Abstract
The technique of gas chromatography-mass spectroscopy was used to identify dihydrodigoxin, a metabolite of digoxin in human plasma and urine. After extraction from urine and plasma, dihydrodigoxin and digoxin were derivatized with heptafluorobutyric anhydride to form the bisheptafluorobutyrate esters. The derivatives can be used for identification by gas chromatography-mass spectroscopy or quantitation at the subnanogram level by gas chromatography with electron capture detection. In the present study, dihydrodigoxin was found to be the major dihydro metabolite of digoxin. Possible clinical implications and utility of the assay are discussed.
Footnotes
- Received July 10, 1972.
- Accepted October 11, 1972.
- © 1973 by The Williams & Wilkins Co.
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