Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleArticle

ENTRY OF LABELED CARBACHOL IN BRAIN SLICES OF THE RAT AND THE ACTION OF d-TUBOCURARINE AND STRYCHNINE

H. Creese and D. B. Taylor
Journal of Pharmacology and Experimental Therapeutics August 1967, 157 (2) 406-419;
H. Creese
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. B. Taylor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Slides of cerebral cortex, cereheilar cortex and gray matter of time spinal cord of rats concentrated carhachol wimicim had been labeled with tritium, and the uptake was largely inhibited by d-tubocurarine and by strychnine. White matter showed little accumulation. Carbachol up to 0.8 M produced no detectable loss of potassium in slices of cerebral cortex, and at low doses the carbachol became concentrated until the ratio between tissue and saline approached that of Potassium. In the absence of glucose there was a fall in potassium content and a low uptake of carhachol, and with high external potassium the tissues were unable to concentrate either potassium or carbachol. With increased concentrations of carbachol time accumulation of the drug showed saturation, and the movements of carbachol were consistent with a monovalent carrier mechanism having a half-saturation constant of 41 µM and a capacity of 156 x 10-9 mol/g/hr. The saturable and curare-sensitive components of entry were similar, and the saturable entry of carhachol was inhibited by d-tubocurarine with an inhibitory constant of 3 µM. The effect of d-tubocurarine in slowing the influx of labeled carbachol could he described by time same procedures as those used in time case of pharmacologic antagonism, and the dose-effect curve and Gaddum's equation for equinctive doses were found to apply to the inward movements of carbachol.

Footnotes

    • Received September 12, 1966.
    • Accepted March 16, 1967.
  • © 1967 by The Williams & Wilkins Company

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 157, Issue 2
1 Aug 1967
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
ENTRY OF LABELED CARBACHOL IN BRAIN SLICES OF THE RAT AND THE ACTION OF d-TUBOCURARINE AND STRYCHNINE
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

ENTRY OF LABELED CARBACHOL IN BRAIN SLICES OF THE RAT AND THE ACTION OF d-TUBOCURARINE AND STRYCHNINE

H. Creese and D. B. Taylor
Journal of Pharmacology and Experimental Therapeutics August 1, 1967, 157 (2) 406-419;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

ENTRY OF LABELED CARBACHOL IN BRAIN SLICES OF THE RAT AND THE ACTION OF d-TUBOCURARINE AND STRYCHNINE

H. Creese and D. B. Taylor
Journal of Pharmacology and Experimental Therapeutics August 1, 1967, 157 (2) 406-419;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • PST3093 Stimulates SERCA2a and Improves Cardiac Function
  • CRV431 Decreases Liver Fibrosis and Tumor Development
  • Discriminative Stimulus Effects of Zolpidem in Squirrel Monkeys: Comparison with Conventional Benzodiazepines and Sedative-Hypnotics
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics