Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Pharmacology and Experimental Therapeutics
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Journal of Pharmacology and Experimental Therapeutics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit jpet on Facebook
  • Follow jpet on Twitter
  • Follow jpet on LinkedIn
Research ArticleArticle

THE BLOCKING ACTION OF HEXAFLUORENIUM ON NEUROMUSCULAR TRANSMISSION AND ITS INTERACTION WITH SUCCINYLCHOLINE

W. L. Nastuk and J. H. Karis
Journal of Pharmacology and Experimental Therapeutics May 1964, 144 (2) 236-252;
W. L. Nastuk
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. H. Karis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The action of hexafluorenium (HFL) and certain other agents has been studied using the sartorius muscle-sciatic nerve preparation of the frog. Indirect stimulation was used to produce muscle twitches.

In the indirectly stimulated muscle, HFL reduces the twitch tension without preceding twitch potentiation. The twitch depression is reversible; it amounts to 10% with HFL 5 x 10-6 M and it increases to 100% with HFL 30 x 10-6 M. In terms of twitch tension reduction HFL is approximately 15x weaker than d-tubocurarine (dTC).

The fall in twitch tension produced by HFL 20 x 10-6 M is not antagonized by edrophonium (lO to 5O x 1O-6 M).

HFL (10 x 10-6 M) causes marked inhibition of the depolarizing action of acetyicholine on the postjunctional membrane.

HFL (10 x 10-6 M) does not cause any change in the resting potential of the postjunctional membrane. This concentration of HFL produces an increase in the amplitude and rate of repolarization of action potentials neurally initiated at the endplate. The maximum rate of rise of the endplate potential is significantly reduced. No significant change in the critical membrane potential occurs.

In junctions where transmission is blocked by HFL (10 to 30 x 10-6 M) the endplate potential is reduced in amplitude and its time course parallels that seen at junctions blocked with d-tubocurarine.

In preparations treated with HFL (5 x 10-6 M) addition of succinylcholine (SCh) (1.25 to 5 x 10-6 M) and carbamyicholine (11 x 10-6 M) causes a fall in twitch tension with little or no preceding twitch potentiation.

In preparations treated with fluorenyltri-methylammonium (FTMA) (50 x 10-6 M) addition of SCh (5 x 10-6 M) causes a fall in twitch tension which is preceded by a small twitch potentiation.

In preparations treated with dTC (0.36 x 10-6 M) the twitch tension falls about 15%. Addition of SCh (5 x 10-6 M) increases the twitch tension to above control levels.

A fall in twitch tension produced by HFL (5 x 10-6 M) plus SCh (2.5 x 10-6 M) is not antagonized by edrophonium (50 x 10-6 M).

In certain respects the neuromuscular block produced by HFL superficially resembles that produced by dTC but other evidence indicates that these agents do not act in a parallel manner. A scheme is presented in which it is suggested that HFL blocks the mechanism by which the postjunctional membrane becomes depolarized when receptor sites become activated by agents such as ACh. Our results help to explain the clinical observation that HFL augments the neuromuscular block produced by intravenously administered SCh.

Footnotes

    • Received July 11, 1963.
    • Accepted December 18, 1963.
  • The Williams & Wilkins Company

JPET articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Journal of Pharmacology and Experimental Therapeutics
Vol. 144, Issue 2
1 May 1964
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Journal of Pharmacology and Experimental Therapeutics article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
THE BLOCKING ACTION OF HEXAFLUORENIUM ON NEUROMUSCULAR TRANSMISSION AND ITS INTERACTION WITH SUCCINYLCHOLINE
(Your Name) has forwarded a page to you from Journal of Pharmacology and Experimental Therapeutics
(Your Name) thought you would be interested in this article in Journal of Pharmacology and Experimental Therapeutics.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

THE BLOCKING ACTION OF HEXAFLUORENIUM ON NEUROMUSCULAR TRANSMISSION AND ITS INTERACTION WITH SUCCINYLCHOLINE

W. L. Nastuk and J. H. Karis
Journal of Pharmacology and Experimental Therapeutics May 1, 1964, 144 (2) 236-252;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

THE BLOCKING ACTION OF HEXAFLUORENIUM ON NEUROMUSCULAR TRANSMISSION AND ITS INTERACTION WITH SUCCINYLCHOLINE

W. L. Nastuk and J. H. Karis
Journal of Pharmacology and Experimental Therapeutics May 1, 1964, 144 (2) 236-252;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • PST3093 Stimulates SERCA2a and Improves Cardiac Function
  • CRV431 Decreases Liver Fibrosis and Tumor Development
  • Discriminative Stimulus Effects of Zolpidem in Squirrel Monkeys: Comparison with Conventional Benzodiazepines and Sedative-Hypnotics
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About JPET
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0103 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics