Abstract
1. A fluorometric method for the determination of serotonin (5-hydroxytryp-tamine) in brain is described. The procedure involves the use of a spectrophoto-fluorometer which can activate and measure emitted fluorescence continuously from 250 to 650 mµ. Serotonin is activated maximally at 295 mµ and emits fluorescent light which shows a maximum at 550 mµ.
2. With this method serotonin has been shown to be a normal constituent of brain.
3. The fluorescent procedure may be applied to the measurement of serotonin in other tissues.
Footnotes
- Received December 12, 1955.
JPET articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
In this issue
IDENTIFICATION AND ASSAY OF SEROTONIN IN BRAIN
