Abstract
The second messenger cAMP is involved in a number of cellular signaling pathways. In mammals, cAMP is produced by either the hormonally responsive, G protein–regulated transmembrane adenylyl cyclases (tmACs) or by the bicarbonate- and calcium-regulated soluble adenylyl cyclase (sAC). To develop tools to differentiate tmAC and sAC signaling, we determined the specificity and potency of commercially available adenylyl cyclase inhibitors. In cellular systems, two inhibitors, KH7 and catechol estrogens, proved specific for sAC, and 2′,5′-dideoxyadenosine proved specific for tmACs. These tools provide a means to define the specific contributions of the different families of adenylyl cyclases in cells and tissues, which will further our understanding of cell signaling.
Footnotes
- Received July 3, 2013.
- Accepted October 2, 2013.
↵1 Current affiliation: Laboratory of Obesity and Aging Research, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland.
J.L.B. and L.R.-E. contributed equally.
This work was supported by the National Institutes of Health National Institute of General Medicine [Grant GM62328]; and the National Institutes of Health Eunice Kennedy Shriver National Institute of Child Health and Human Development [Grant HD059913].
- Copyright © 2013 by The American Society for Pharmacology and Experimental Therapeutics
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