Structure and function of G protein-coupled receptors

CD Strader, TM Fong, MR Tota… - Annual review of …, 1994 - annualreviews.org
A variety of cell-surface receptors mediate their intracellular actions by a pathway that involves
activation of one or more guanine nucleotide-binding regulatory proteins (G proteins). …

The family of G‐protein‐coupled receptors

…, TM Fong, MP Grazlano, MR Tota - The FASEB …, 1995 - Wiley Online Library
TOTA Department of Molecular Pharmacology and Biochemistry, Merck Research
Laboratories, Rahway, New Jersey 07065, USA … MP, Tota, MR The family of G-protein-coupled …

A role for the melanocortin 4 receptor in sexual function

…, TM Fong, C Huang, MR Tota… - Proceedings of the …, 2002 - National Acad Sciences
… Specific binding was defined as the difference between total binding and nonspecific
binding assayed in 1,000 nM MTII. Specific binding was 40–50% of total binding. Assays were …

ART (protein product of agouti-related transcript) as an antagonist of MC-3 and MC-4 receptors

…, R Kalyani, T Smith, D Weinberg, MR Tota… - Biochemical and …, 1997 - Elsevier
Tota, and Lex HT Van der Ploeg … NDP-a-MSH), varying concentrations of ART, and an
appropriate amount of membranes so that the total bound radioligand was less …

Localization of leptin binding domain in the leptin receptor

TM Fong, RRC Huang, MR Tota, C Mao, T Smith… - Molecular …, 1998 - ASPET
… a much more robust luciferase response than that observed in COS cell transfection, thereby
compensating the reduced protein expression level due to increased amounts of total DNA. …

[HTML][HTML] Liver and adipose expression associated SNPs are enriched for association to type 2 diabetes

…, K Hao, S Woo, C Fabre-Suver, S Qian, MR Tota… - PLoS …, 2010 - journals.plos.org
… The adipose tissues were homogenized and total RNA extracted using Trizol reagent (Invitrogen,
CA). Three micrograms of total RNA was reverse transcribed and labeled with either …

Antidiabetic and antisteatotic effects of the selective fatty acid synthase (FAS) inhibitor platensimycin in mouse models of diabetes

…, C Miller, RW Myers, MR Tota… - Proceedings of the …, 2011 - National Acad Sciences
Platensimycin (PTM) is a recently discovered broad-spectrum antibiotic produced by
Streptomyces platensis. It acts by selectively inhibiting the elongation-condensing enzyme FabF of …

Molecular determinants of ligand binding to the human melanocortin-4 receptor

…, TM Fong, CJ Dickinson, C Mao, JY Li, MR Tota… - Biochemistry, 2000 - ACS Publications
… the amount of 125 I-label remaining bound in the presence of 10 - 5 M unlabeled ligand,
and specific binding was obtained by subtracting nonspecific bound radioactivity from total

Crystal structure of human phosphodiesterase 3B: atomic basis for substrate and inhibitor specificity

…, JW Becker, LHT Van der Ploeg, MR Tota - Biochemistry, 2004 - ACS Publications
… The final search model contained 243 residues out of the total of 420 (58% of the model).
The molecular replacement solution (all four molecules) had a correlation coefficient of 0.224% …

Molecular interaction of Agouti protein and Agouti-related protein with human melanocortin receptors

MR Tota, TS Smith, C Mao, T MacNeil, RT Mosley… - Biochemistry, 1999 - ACS Publications
Agouti protein and the Agouti-related protein (AGRP) are antagonists of the melanocortin-3
receptor and melanocortin-4 receptor. Both proteins contain 10 cysteines in the C-terminal …