… ml/100 ml medium). All cells were maintained at 37C under a humidified, 5% CO 2 atmosphere.
Cells were routinely subcultured by trypsinization (0.25% trypsin, 0.03% EDTA; Gibco, …

Cancer chemotherapy has evolved from the use of cytotoxic drugs that are accompanied by
highly deleterious and often life-threatening side effects, to the application of hormone …

… , we have demonstrated that in the absence of SIRT1, E1–E2 replication is extremely
mutagenic and the E1–E2 DNA replication foci are disrupted in the absence of SIRT1 (Das, Bristol …

… (0.5 mL/100 mL medium). Hs578t cells were grown from frozen stocks in α-DMEM
supplemented with 10% FCS, 2 mmol/L l-glutamine and penicillin/streptomycin (0.5 mL/100 mL …

… For quantification of AVOs, treated cells were trypsinized, harvested, and washed with PBS
at approximately 1 × 10 6 cells/ml. Pellets were resuspended in PBS and stained with a final …

… The latter were selected in medium with 1 μg/mL puromycin to enrich the infected cells. A
70… The transduced cells were selected within medium with 1 μg/mL puromycin to obtain stable …

… Xenograft Studies Nu/Nu mice were injected with 5 3 106 cells/100 ml of PBS, in combination
with 100 ml of Matrigel. Mice were injected subcutaneously and monitored for tumor growth…

… Transfected cells were selected with 150 μg/ml G418 (Corning), with the medium changed
… -1, and N/Tert-1+E2 pools were grown in medium containing 5 μg/ml puromycin (Corning). …

High risk-human papillomaviruses (HPVs) are known carcinogens. Numerous reports have
linked the steroid hormone estrogen, and the expression of estrogen receptors (ERs), to HPV…

… Cells containing wild-type or mutant HPV16 were grown in the same medium but also
containing 150 μg/ml G418 (Thermo Fisher Scientific). All cells were routinely checked for …