The purpose of this position paper is to present a critical analysis of the challenges and
limitations of the most widely used fluorescent probes for detecting and measuring reactive …

Nitric oxide (NO) methodology is a complex and often confusing science and the focus of
many debates and discussion concerning NO biochemistry. NO is involved in many …

… Miles AM, Bohle DS, Glassbrenner PA, Hansert B, Wink DA, Grisham MB. Modulation of
superoxide-dependent oxidation and hydroxylation reactions by nitric oxide. J Biol Chem 1996; …

… Briefly, neutrophils and mononuclear leukocytes were purified by the method of Grisham
et al. (8) in which erythrocytes were sedimented with dextran, and mononuclear and …

… Feline neutrophils were purified according to the method of Grisham et al. (7). Briefly, cells
from 30 ml of … GRISHAM, MB, MM JEFFERSON, AND EL THOMAS. Role of monochloramine …

Facile detection of reactive oxygen and nitrogen species in biologic systems is often
problematic. This is a result of the numerous cellular mechanisms, both enzymatic and …

… activated would produce 100–1,000 times more O 2 − than NO, suggesting that PMNs
would produce little or no ONOO − /ONOOH and indeed this is exactly what we observe (Grisham …

Focuses on the role of oxidative stress in intestinal injury and dysfunction associated with
inflammatory bowel disease. Activity of leucocyte-derived oxidants; Overproduction of reactive …

… Grisham MB, McCord JM: Chemistry and cytotoxicity of reactive oxygen metabolites. In
Physiology of Oxygen Radicals, AE Taylor, S Matalon, PA Ward (eds). Bethesda, American …

Isolated human neutrophilic leukocytes were stimulated to produce hydrogen peroxide (H2O2)
and to secrete cytoplasmic granule components including myeloperoxidase into the …